PCR-RFLP analysis of Giardia intestinalis using a Giardia-specific gene, GLORF-C4

T. Yong, K. Han, H. Yang, Soon Jung Park

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12 Citations (Scopus)

Abstract

A cDNA clone encoding GLORF-C4 was isolated from the WB strain, an assemblage A Giardia intestinalis. Interestingly, GLORF-C4 has been previously reported as an assemblage B-specific gene. Using two primers based on GLORF-C4 of the GS strain, a prototype assemblage B, GLORF-C4 gene was amplified from ail the groups of G. intestinalis, and applied to detect the presence of cysts of G. intestinalis from faecal samples of cyst-passers. RFLP analysis of this PCR product successfully classified G. intestinalis into two distinct groups, assemblages A and B.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalParasite
Volume9
Issue number1
DOIs
Publication statusPublished - 2002 Mar

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All Science Journal Classification (ASJC) codes

  • Parasitology
  • Animal Science and Zoology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

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