PCR-RFLP analysis of Giardia intestinalis using a Giardia-specific gene, GLORF-C4

Taisoon Yong, KwangHyub Han, H. Yang, Soon Jung Park

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A cDNA clone encoding GLORF-C4 was isolated from the WB strain, an assemblage A Giardia intestinalis. Interestingly, GLORF-C4 has been previously reported as an assemblage B-specific gene. Using two primers based on GLORF-C4 of the GS strain, a prototype assemblage B, GLORF-C4 gene was amplified from ail the groups of G. intestinalis, and applied to detect the presence of cysts of G. intestinalis from faecal samples of cyst-passers. RFLP analysis of this PCR product successfully classified G. intestinalis into two distinct groups, assemblages A and B.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalParasite
Volume9
Issue number1
DOIs
Publication statusPublished - 2002 Jan 1

Fingerprint

Giardia
Giardia lamblia
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
Polymerase Chain Reaction
Genes
Cysts
genes
Passer
prototypes
Complementary DNA
Clone Cells
clones
sampling

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Animal Science and Zoology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

Cite this

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PCR-RFLP analysis of Giardia intestinalis using a Giardia-specific gene, GLORF-C4. / Yong, Taisoon; Han, KwangHyub; Yang, H.; Park, Soon Jung.

In: Parasite, Vol. 9, No. 1, 01.01.2002, p. 65-70.

Research output: Contribution to journalArticle

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