Penetration pathways induced by low-frequency sonophoresis with physical and chemical enhancers

Iron oxide nanoparticles versus lanthanum nitrates

Sang Eun Lee, Ki Ju Choi, Gopinathan K. Menon, Hyun Jung Kim, Eung Ho Choi, Sung Ku Ahn, Seung Hun Lee

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Low-frequency sonophoresis (LFS) has been shown to disrupt the structure of stratum corneum (SC) lipid bilayers and enhance SC permeability. In this study, we examined the penetration pathway of lanthanum nitrate (LaNO 3 ) tracer in viable epidermis after combined treatment of LFS and tape stripping (TS), as a physical enhancer, or oleic acid (OA) application, as a chemical enhancer, using transmission electron microscopy (TEM). As a positive control, we visualized the passive diffusion pathway of LaNO 3 and iron oxide (Fe 3 O 4 ) nanoparticles after the incision of hairless mouse skin. Next, we applied LFS immediately after TS or OA application and visualized the penetration pathway of LaNO 3 . Each treatment showed restricted penetration to the SC-stratum granulosum (SG) interface or upper SG layer. However, the additional application of LFS induced diffuse intracellular distribution of LaNO 3 throughout the viable epidermis. Quantitative analysis also revealed that combined treatment significantly increases LaNO 3 penetration into viable epidermis when compared with each treatment. Our ultrastructural findings show the synergistic effect of LFS and TS or OA application on transdermal drug delivery. We also found that this combined treatment enhances the penetration of LaNO 3 through the viable epidermis through an intracellular pathway.

Original languageEnglish
Pages (from-to)1063-1072
Number of pages10
JournalJournal of Investigative Dermatology
Volume130
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

Epidermis
Nanoparticles
Oleic Acid
Cornea
Tapes
Hairless Mouse
Lipid bilayers
Lipid Bilayers
Transmission Electron Microscopy
Drug delivery
Permeability
Skin
Transmission electron microscopy
lanthanide nitrate
ferric oxide
Chemical analysis
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Lee, Sang Eun ; Choi, Ki Ju ; Menon, Gopinathan K. ; Kim, Hyun Jung ; Choi, Eung Ho ; Ahn, Sung Ku ; Lee, Seung Hun. / Penetration pathways induced by low-frequency sonophoresis with physical and chemical enhancers : Iron oxide nanoparticles versus lanthanum nitrates. In: Journal of Investigative Dermatology. 2010 ; Vol. 130, No. 4. pp. 1063-1072.
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Penetration pathways induced by low-frequency sonophoresis with physical and chemical enhancers : Iron oxide nanoparticles versus lanthanum nitrates. / Lee, Sang Eun; Choi, Ki Ju; Menon, Gopinathan K.; Kim, Hyun Jung; Choi, Eung Ho; Ahn, Sung Ku; Lee, Seung Hun.

In: Journal of Investigative Dermatology, Vol. 130, No. 4, 01.04.2010, p. 1063-1072.

Research output: Contribution to journalArticle

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