Peptide nucleic acid-mediated aggregation of reduced graphene oxides and label-free detection of DNA mutation

Taegyeong Kang, Hoon Choi, Sang Woo Joo, So Yeong Lee, Kyong Ah Yoon, Kangtaek Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have investigated peptide nucleic acid (PNA)-mediated aggregation of reduced graphene oxide (rGO) sheets. Addition of PNA into suspension of rGOs resulted in aggregation of rGOs, which could be easily detected with the naked eye. To elucidate the mechanism of rGO aggregation, we monitored the reaction by transmission electron microscopy, zeta potential measurement, and UV-vis spectroscopy. Our findings suggest that PNA adsorbed on the rGO surface and then acted as a cross-linker to induce aggregation. We also tested the effects of different nucleic acids on rGO aggregation and found that not only the single-stranded DNA, but also the PNA-DNA complex, could stabilize the suspension against aggregation through electrostatic repulsion. Based on our understanding on rGO aggregation, we attempted to detect mutations in epidermal growth factor receptor (EGFR) by designing PNA probe to be complementary to the mutant type DNA sequence. Our results showed that PNA-mediated rGO aggregation could successfully be used to detect mutations in EGFR with high selectivity and sensitivity.

Original languageEnglish
Pages (from-to)6297-6301
Number of pages5
JournalJournal of Physical Chemistry B
Volume118
Issue number23
DOIs
Publication statusPublished - 2014 Jun 12

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All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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