Atopic dermatitis (AD) is a chronic inflammatory skin disease whose prevalence is increasing worldwide. Filaggrin (FLG) is essential for the development of the skin barrier, and its genetic mutations are major predisposing factors for AD. In this study, we developed a convenient and practical method to detect FLG mutations in AD patients using peptide nucleic acid (PNA) probes labelled with fluorescent markers for rapid analysis. Fluorescence melting curve analysis (FMCA) precisely identified FLG mutations based on the distinct difference in the melting temperatures of the wild-type and mutant allele. Moreover, PNA probe-based FMCA easily and accurately verified patient samples with both heterozygote and homozygote FLG mutations, providing a high-throughput method to reliable screen AD patients. Our method provides a convenient, rapid and accurate diagnostic tool to identify potential AD patients allowing for early preventive treatment, leading to lower incidence rates of AD, and reducing total healthcare expenses.
Bibliographical noteFunding Information:
This work was supported by the R&D Convergence Program (CAP-16-03-KRIBB, BY Kim) and the International Cooperation Research Program (JS Ahn) of National Research Council of Science & Technology (NST) of Republic of Korea. This work was also supported by the Bio and Medical Technology Development Program (NRF-2014M3A9B5073938, BY Kim), the Basic Science Research Program (NRF-2016R1A2B3011389, YT Kwon), and MRC (2017R1A5A2015541, JT Hong) of the Ministry of Science and ICT (MSIT) of Korea, and KRIBB Research Initiative Program. We thank Mr. Molstad M. for his support in manuscript proofreading.
R&D Convergence Program, Grant/ Award Number: CAP-16-03-KRIBB, and International Cooperation Research Program of National Research Council of Science & Technology (NST) of Republic of Korea; Bio and Medical Technology Development Program, Grant/Award
Number: NRF-2014M3A9B5073938; Basic Science Research Program, Grant/Award Number: MRC (2017R1A5A2015541) and NRF-2016R1A2B3011389; the Ministry of Science and ICT (MSIT) of Korea; KRIBB Research Initiative Program
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All Science Journal Classification (ASJC) codes
- Molecular Biology