Performance of hollow-fiber flow field-flow fractionation in protein separation

Ilyong Park, Ki Jung Paeng, Dukjin Kang, Myeong Hee Moon

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Since hollow-fiber flow field-flow fractionation (HF FIFFF) utilizes a cylindrical channel made of a hollow-fiber membrane, which is inexpensive and simple in channel assembly and thus disposable, interests are increasing as a potential separation device in cells, proteins, and macromolecules. In this study, performance of HF FIFFF of proteins is described by examining the influence of flow rate conditions and length of fiber (polyacrylonitrile or PAN in this work) on sample recovery as well as experimental plate heights. The interfiber reproducibility in terms of separation time and recovery was also studied. Experiments showed that sample recovery was consistent regardless of the length of fiber when the effective field strength (equivalent to the mean flow velocity at the fiber wall) and the channel void time were adjusted to be equivalent for channels of various fiber lengths. This supported that the majority of sample loss in HF FIFFF separation of apoferritin and their aggregates may occur before the migration process. It is finally demonstrated that HF FIFFF can be applied for characterizing the reduction in Stokes' size of low density lipoproteins from blood plasma samples obtained from patients having coronary artery disease and from healthy donors.

Original languageEnglish
Pages (from-to)2043-2049
Number of pages7
JournalJournal of Separation Science
Volume28
Issue number16
DOIs
Publication statusPublished - 2005 Nov

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Fingerprint Dive into the research topics of 'Performance of hollow-fiber flow field-flow fractionation in protein separation'. Together they form a unique fingerprint.

Cite this