Performance of the BacT alert 3D system versus solid media for recovery and drug susceptibility testing of Mycobacterium tuberculosis in a tertiary hospital in Korea

Seoung Cheol Kim, Bo Young Jeon, Jin Sook Kim, In Hwan Choi, Jiro Kim, Jeongim Woo, Soojin Kim, Hyeong Woo Lee, Monoldorova Sezim, Sang Nae Cho

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. Methods: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on Löwenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. Results: The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%). Conclusion: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.

Original languageEnglish
Pages (from-to)282-288
Number of pages7
JournalTuberculosis and Respiratory Diseases
Volume79
Issue number4
DOIs
Publication statusPublished - 2016 Oct

Fingerprint

Korea
Mycobacterium tuberculosis
Tertiary Care Centers
Multidrug-Resistant Tuberculosis
Pharmaceutical Preparations
Tuberculosis
Isoniazid
Rifampin
Microbial Drug Resistance
Sputum
Drug Resistance
Health

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

Cite this

Kim, Seoung Cheol ; Jeon, Bo Young ; Kim, Jin Sook ; Choi, In Hwan ; Kim, Jiro ; Woo, Jeongim ; Kim, Soojin ; Lee, Hyeong Woo ; Sezim, Monoldorova ; Cho, Sang Nae. / Performance of the BacT alert 3D system versus solid media for recovery and drug susceptibility testing of Mycobacterium tuberculosis in a tertiary hospital in Korea. In: Tuberculosis and Respiratory Diseases. 2016 ; Vol. 79, No. 4. pp. 282-288.
@article{eb6ad8af019c45ebbc43be9c8b67dbc1,
title = "Performance of the BacT alert 3D system versus solid media for recovery and drug susceptibility testing of Mycobacterium tuberculosis in a tertiary hospital in Korea",
abstract = "Background: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. Methods: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on L{\"o}wenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. Results: The recovery rates were 94.9{\%} (206/217) and 98.2{\%} (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4{\%} for at least one or more drugs, 6.0{\%} for MDR-TB and 2.3{\%} for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7{\%}; kappa coefficient, 0.905) and rifampin (98.6{\%}; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8{\%}). Conclusion: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.",
author = "Kim, {Seoung Cheol} and Jeon, {Bo Young} and Kim, {Jin Sook} and Choi, {In Hwan} and Jiro Kim and Jeongim Woo and Soojin Kim and Lee, {Hyeong Woo} and Monoldorova Sezim and Cho, {Sang Nae}",
year = "2016",
month = "10",
doi = "10.4046/trd.2016.79.4.282",
language = "English",
volume = "79",
pages = "282--288",
journal = "Tuberculosis and Respiratory Diseases",
issn = "1738-3536",
publisher = "The Korean Academy of Tuberculosis and Respiratory Diseases",
number = "4",

}

Performance of the BacT alert 3D system versus solid media for recovery and drug susceptibility testing of Mycobacterium tuberculosis in a tertiary hospital in Korea. / Kim, Seoung Cheol; Jeon, Bo Young; Kim, Jin Sook; Choi, In Hwan; Kim, Jiro; Woo, Jeongim; Kim, Soojin; Lee, Hyeong Woo; Sezim, Monoldorova; Cho, Sang Nae.

In: Tuberculosis and Respiratory Diseases, Vol. 79, No. 4, 10.2016, p. 282-288.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Performance of the BacT alert 3D system versus solid media for recovery and drug susceptibility testing of Mycobacterium tuberculosis in a tertiary hospital in Korea

AU - Kim, Seoung Cheol

AU - Jeon, Bo Young

AU - Kim, Jin Sook

AU - Choi, In Hwan

AU - Kim, Jiro

AU - Woo, Jeongim

AU - Kim, Soojin

AU - Lee, Hyeong Woo

AU - Sezim, Monoldorova

AU - Cho, Sang Nae

PY - 2016/10

Y1 - 2016/10

N2 - Background: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. Methods: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on Löwenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. Results: The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%). Conclusion: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.

AB - Background: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. Methods: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on Löwenstein-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. Results: The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%). Conclusion: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.

UR - http://www.scopus.com/inward/record.url?scp=84994316383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994316383&partnerID=8YFLogxK

U2 - 10.4046/trd.2016.79.4.282

DO - 10.4046/trd.2016.79.4.282

M3 - Article

AN - SCOPUS:84994316383

VL - 79

SP - 282

EP - 288

JO - Tuberculosis and Respiratory Diseases

JF - Tuberculosis and Respiratory Diseases

SN - 1738-3536

IS - 4

ER -