Performance of the Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories

Hee Won Moon, Sun Hwa Lee, Hae Sun Chung, Miae Lee, Kyungwon Lee

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26 Citations (Scopus)

Abstract

We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9% of the isolates tested to species level. The Vitek MS correctly identified the species of 97.2% of the staphylococci (95.9% of coagulasenegative staphylococci), 97.8% of the streptococci, and 100% of the enterococci. For the identification of Gram-positive cocci isolates, the overall concordance rate between conventional identification and the Vitek MS was 94.5 %. The Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system can be a reliable and rapid method for the identification of most relevant Gram-positive cocci. In addition, expanding the database of the Vitek MS, especially for coagulase-negative staphylococci, is needed to enhance the performance of the Vitek MS.

Original languageEnglish
Pages (from-to)1301-1306
Number of pages6
JournalJournal of Medical Microbiology
Volume62
DOIs
Publication statusPublished - 2013 Aug 21

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Gram-Positive Cocci
Microbiology
Staphylococcus
Mass Spectrometry
Lasers
Coagulase
Enterococcus
Streptococcus
rRNA Genes
Databases

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

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title = "Performance of the Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry system for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories",
abstract = "We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9{\%} of the isolates tested to species level. The Vitek MS correctly identified the species of 97.2{\%} of the staphylococci (95.9{\%} of coagulasenegative staphylococci), 97.8{\%} of the streptococci, and 100{\%} of the enterococci. For the identification of Gram-positive cocci isolates, the overall concordance rate between conventional identification and the Vitek MS was 94.5 {\%}. The Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system can be a reliable and rapid method for the identification of most relevant Gram-positive cocci. In addition, expanding the database of the Vitek MS, especially for coagulase-negative staphylococci, is needed to enhance the performance of the Vitek MS.",
author = "Moon, {Hee Won} and Lee, {Sun Hwa} and Chung, {Hae Sun} and Miae Lee and Kyungwon Lee",
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AU - Moon, Hee Won

AU - Lee, Sun Hwa

AU - Chung, Hae Sun

AU - Lee, Miae

AU - Lee, Kyungwon

PY - 2013/8/21

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N2 - We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9% of the isolates tested to species level. The Vitek MS correctly identified the species of 97.2% of the staphylococci (95.9% of coagulasenegative staphylococci), 97.8% of the streptococci, and 100% of the enterococci. For the identification of Gram-positive cocci isolates, the overall concordance rate between conventional identification and the Vitek MS was 94.5 %. The Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system can be a reliable and rapid method for the identification of most relevant Gram-positive cocci. In addition, expanding the database of the Vitek MS, especially for coagulase-negative staphylococci, is needed to enhance the performance of the Vitek MS.

AB - We evaluated the performance of the Vitek MS for identification of Gram-positive cocci routinely isolated in clinical microbiology laboratories. With a total of 424 well-characterized isolates, the results of the Vitek MS were compared to those of conventional methods and 16S rRNA gene sequencing. The Vitek MS correctly identified 97.9% of the isolates tested to species level. The Vitek MS correctly identified the species of 97.2% of the staphylococci (95.9% of coagulasenegative staphylococci), 97.8% of the streptococci, and 100% of the enterococci. For the identification of Gram-positive cocci isolates, the overall concordance rate between conventional identification and the Vitek MS was 94.5 %. The Vitek MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) system can be a reliable and rapid method for the identification of most relevant Gram-positive cocci. In addition, expanding the database of the Vitek MS, especially for coagulase-negative staphylococci, is needed to enhance the performance of the Vitek MS.

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