Perillyl alcohol inhibits the expression and function of the androgen receptor in human prostate cancer cells

Byung Ha Chung, Hye young Lee, Jae Seok Lee, Charles Y.F. Young

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Perillyl alcohol is a hydroxylated monocyclic monoterpene. In animal study, monoterpene has shown to have an anti-tumor effect. The aim of this study is to evaluate whether POH plays an important role in the development and progression of prostate cancer (pCa). We treated LNCaP cells with different concentrations of perillyl alcohol (POH). First of all, we performed cell proliferation assay and prostate-specific antigen (PSA) and human glandular kallikrein (hK2) quantification assays. LNCaP cells were treated with or without POH for Western blot analysis of androgen receptor (AR) and c-Jun. Finally, we performed transient transfection assay by transfecting LNCaP cells-which were treated with or without POH-with pGL-3 luciferase vector containing PSA promoter and AR promoter. We observed inhibition of the expression and function of the AR by POH, through inhibition of androgen-induced cell growth and androgen-stimulated secretion of prostate-specific antigen and hK2, in human pCa cell line LNCaP. In addition, we demonstrated, for the first time, that POH inhibits the transcription activities of the AR gene promoter by over-expression of c-Jun protein. These novel properties of POH strongly suggest that POH could be highly useful for intervention of pCa.

Original languageEnglish
Pages (from-to)222-228
Number of pages7
JournalCancer Letters
Volume236
Issue number2
DOIs
Publication statusPublished - 2006 May 18

Bibliographical note

Funding Information:
Supported by NIH grants NCI/DK89000 and Army Defense grant DAMD17-98-1-8523.

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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