Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor-5 in a β-tricalcium phosphate carrier into one-wall intrabony defects in dogs

Jung Seok Lee, Ulf M.E. Wikesjö, Ui-Won Jung, Seongho Choi, Susanne Pippig, Michael Siedler, Chong Kwan Kim

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following the application of rhGDF-5 on a particulate β-tricalcium phosphate (β-TCP) carrier using an established defect model. Materials and Methods: Bilateral 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in 15 Beagle dogs. Unilateral defects in five animals received rhGDF-5/β-TCP (Scil Technology GmbH); five animals received β-TCP solo; and five animals served as sham-surgery controls. Contralateral sites received treatments reported elsewhere. The animals were sacrificed following an 8-week healing interval for histological examination. Results: Clinical healing was generally uneventful. Sites implanted with rhGDF-5/β-TCP exhibited greater enhanced cementum and bone formation compared with β-TCP and sham-surgery controls; cementum regeneration averaged (± SD) 3.83 ± 0.73 versus 1.65 ± 0.82 and 2.48 ± 1.28 mm for the controls (p<0.05). Corresponding values for bone regeneration height averaged 3.26 ± 0.30 versus 1.70 ± 0.66 and 1.68 ± 0.49 mm (p<0.05), and bone area 10.45 ± 2.26 versus 6.31 ± 2.41 and 3.00 ± 1.97 mm2 (p<0.05). Cementum regeneration included cellular/acellular cementum with or without a functionally oriented periodontal ligament. A non-specific connective tissue attachment was evident in the sham-surgery control. Controls exhibited mostly woven bone with primary osteons, whereas rhGDF-5/β-TCP sites showed a noticeable extent of lamellar bone. Sites receiving rhGDF-5/β-TCP or β-TCP showed some residual β-TCP granules apparently undergoing biodegradation without obvious differences between the sites. Sites receiving β-TCP alone commonly showed residual β-TCP granules sequestered in the connective tissue or fibrovascular marrow. Conclusion: rhGDF-5/β-TCP has a greater potential to support the regeneration of the periodontal attachment. Long-term studies are necessary to confirm the uneventful maturation of the regenerated tissues.

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalJournal of Clinical Periodontology
Volume37
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1

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Phosphate Transport Proteins
Wound Healing
Regeneration
Dental Cementum
Dogs
Bone and Bones
Connective Tissue
Haversian System
Supernumerary Tooth
Periodontal Ligament
Bone Regeneration
human GDF5 protein
tricalcium phosphate
Osteogenesis
Tooth
Bone Marrow
Technology

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

@article{8f1f546c043d4266abfd627f7d28b27e,
title = "Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor-5 in a β-tricalcium phosphate carrier into one-wall intrabony defects in dogs",
abstract = "Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following the application of rhGDF-5 on a particulate β-tricalcium phosphate (β-TCP) carrier using an established defect model. Materials and Methods: Bilateral 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in 15 Beagle dogs. Unilateral defects in five animals received rhGDF-5/β-TCP (Scil Technology GmbH); five animals received β-TCP solo; and five animals served as sham-surgery controls. Contralateral sites received treatments reported elsewhere. The animals were sacrificed following an 8-week healing interval for histological examination. Results: Clinical healing was generally uneventful. Sites implanted with rhGDF-5/β-TCP exhibited greater enhanced cementum and bone formation compared with β-TCP and sham-surgery controls; cementum regeneration averaged (± SD) 3.83 ± 0.73 versus 1.65 ± 0.82 and 2.48 ± 1.28 mm for the controls (p<0.05). Corresponding values for bone regeneration height averaged 3.26 ± 0.30 versus 1.70 ± 0.66 and 1.68 ± 0.49 mm (p<0.05), and bone area 10.45 ± 2.26 versus 6.31 ± 2.41 and 3.00 ± 1.97 mm2 (p<0.05). Cementum regeneration included cellular/acellular cementum with or without a functionally oriented periodontal ligament. A non-specific connective tissue attachment was evident in the sham-surgery control. Controls exhibited mostly woven bone with primary osteons, whereas rhGDF-5/β-TCP sites showed a noticeable extent of lamellar bone. Sites receiving rhGDF-5/β-TCP or β-TCP showed some residual β-TCP granules apparently undergoing biodegradation without obvious differences between the sites. Sites receiving β-TCP alone commonly showed residual β-TCP granules sequestered in the connective tissue or fibrovascular marrow. Conclusion: rhGDF-5/β-TCP has a greater potential to support the regeneration of the periodontal attachment. Long-term studies are necessary to confirm the uneventful maturation of the regenerated tissues.",
author = "Lee, {Jung Seok} and Wikesj{\"o}, {Ulf M.E.} and Ui-Won Jung and Seongho Choi and Susanne Pippig and Michael Siedler and Kim, {Chong Kwan}",
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Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor-5 in a β-tricalcium phosphate carrier into one-wall intrabony defects in dogs. / Lee, Jung Seok; Wikesjö, Ulf M.E.; Jung, Ui-Won; Choi, Seongho; Pippig, Susanne; Siedler, Michael; Kim, Chong Kwan.

In: Journal of Clinical Periodontology, Vol. 37, No. 4, 01.04.2010, p. 382-389.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Periodontal wound healing/regeneration following implantation of recombinant human growth/differentiation factor-5 in a β-tricalcium phosphate carrier into one-wall intrabony defects in dogs

AU - Lee, Jung Seok

AU - Wikesjö, Ulf M.E.

AU - Jung, Ui-Won

AU - Choi, Seongho

AU - Pippig, Susanne

AU - Siedler, Michael

AU - Kim, Chong Kwan

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following the application of rhGDF-5 on a particulate β-tricalcium phosphate (β-TCP) carrier using an established defect model. Materials and Methods: Bilateral 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in 15 Beagle dogs. Unilateral defects in five animals received rhGDF-5/β-TCP (Scil Technology GmbH); five animals received β-TCP solo; and five animals served as sham-surgery controls. Contralateral sites received treatments reported elsewhere. The animals were sacrificed following an 8-week healing interval for histological examination. Results: Clinical healing was generally uneventful. Sites implanted with rhGDF-5/β-TCP exhibited greater enhanced cementum and bone formation compared with β-TCP and sham-surgery controls; cementum regeneration averaged (± SD) 3.83 ± 0.73 versus 1.65 ± 0.82 and 2.48 ± 1.28 mm for the controls (p<0.05). Corresponding values for bone regeneration height averaged 3.26 ± 0.30 versus 1.70 ± 0.66 and 1.68 ± 0.49 mm (p<0.05), and bone area 10.45 ± 2.26 versus 6.31 ± 2.41 and 3.00 ± 1.97 mm2 (p<0.05). Cementum regeneration included cellular/acellular cementum with or without a functionally oriented periodontal ligament. A non-specific connective tissue attachment was evident in the sham-surgery control. Controls exhibited mostly woven bone with primary osteons, whereas rhGDF-5/β-TCP sites showed a noticeable extent of lamellar bone. Sites receiving rhGDF-5/β-TCP or β-TCP showed some residual β-TCP granules apparently undergoing biodegradation without obvious differences between the sites. Sites receiving β-TCP alone commonly showed residual β-TCP granules sequestered in the connective tissue or fibrovascular marrow. Conclusion: rhGDF-5/β-TCP has a greater potential to support the regeneration of the periodontal attachment. Long-term studies are necessary to confirm the uneventful maturation of the regenerated tissues.

AB - Objective: Recombinant human growth/differentiation factor-5 (rhGDF-5) is being evaluated as a candidate therapy in support of periodontal regeneration. The objective of this study was to evaluate periodontal wound healing/regeneration following the application of rhGDF-5 on a particulate β-tricalcium phosphate (β-TCP) carrier using an established defect model. Materials and Methods: Bilateral 4 × 5 mm (width × depth), one-wall, critical-size, intrabony periodontal defects were surgically created at the mandibular second and fourth pre-molar teeth in 15 Beagle dogs. Unilateral defects in five animals received rhGDF-5/β-TCP (Scil Technology GmbH); five animals received β-TCP solo; and five animals served as sham-surgery controls. Contralateral sites received treatments reported elsewhere. The animals were sacrificed following an 8-week healing interval for histological examination. Results: Clinical healing was generally uneventful. Sites implanted with rhGDF-5/β-TCP exhibited greater enhanced cementum and bone formation compared with β-TCP and sham-surgery controls; cementum regeneration averaged (± SD) 3.83 ± 0.73 versus 1.65 ± 0.82 and 2.48 ± 1.28 mm for the controls (p<0.05). Corresponding values for bone regeneration height averaged 3.26 ± 0.30 versus 1.70 ± 0.66 and 1.68 ± 0.49 mm (p<0.05), and bone area 10.45 ± 2.26 versus 6.31 ± 2.41 and 3.00 ± 1.97 mm2 (p<0.05). Cementum regeneration included cellular/acellular cementum with or without a functionally oriented periodontal ligament. A non-specific connective tissue attachment was evident in the sham-surgery control. Controls exhibited mostly woven bone with primary osteons, whereas rhGDF-5/β-TCP sites showed a noticeable extent of lamellar bone. Sites receiving rhGDF-5/β-TCP or β-TCP showed some residual β-TCP granules apparently undergoing biodegradation without obvious differences between the sites. Sites receiving β-TCP alone commonly showed residual β-TCP granules sequestered in the connective tissue or fibrovascular marrow. Conclusion: rhGDF-5/β-TCP has a greater potential to support the regeneration of the periodontal attachment. Long-term studies are necessary to confirm the uneventful maturation of the regenerated tissues.

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