Peripheral CD300a+CD8+ T Lymphocytes with a Distinct Cytotoxic Molecular Signature Increase in Pregnant Women with Chronic Chorioamnionitis

Yi Xu, Federica Tarquini, Roberto Romero, Chong Jai Kim, Adi L. Tarca, Gaurav Bhatti, Joonho Lee, I. Birgitta Sundell, Pooja Mittal, Juan Pedro Kusanovic, Sonia S. Hassan, Jung Sun Kim

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Problem CD300a is an immunomodulatory molecule of the immunoglobulin receptor superfamily expressed in the leukocytes of myeloid and lymphoid lineages. However, its biological function on CD8+ T lymphocytes remains largely unknown. This study was conducted to assess the biological significance of CD300a expression in T lymphocytes and to determine whether its expression in peripheral T lymphocytes changes in pregnant women presenting with antifetal rejection. Methods of Study Microarray analysis was performed using total RNA isolated from peripheral CD300a+ and CD300a- T lymphocytes. Flow cytometric analysis of the peripheral blood samples of pregnant women and pathologic examination of the placentas were conducted. Results A large number of genes (N=1245) were differentially expressed between CD300a- and CD300a+ subsets of CD8+ T lymphocytes, which included CCR7, CD244, CX3CR1, GLNY, GZMB, GZMK, IL15, ITGB1, KLRG1, PRF1, and SLAMF7. Gene ontology analysis of differentially expressed genes demonstrated enrichment of biological processes such as immune response, cell death, and signal transduction. CD300a expression in CD8+ T lymphocytes was coupled to a more cytotoxic molecular signature. Of note, the proportion of CD300a+CD8+ T lymphocytes increased in pregnant women with chronic chorioamnionitis (antifetal rejection of the chorioamniotic membranes; P<0.05). Conclusion The findings of this study strongly suggest an increase in systemic T-lymphocyte-mediated cytotoxicity in pregnant women with chronic chorioamnionitis as a manifestation of maternal antifetal rejection.

Original languageEnglish
Pages (from-to)184-197
Number of pages14
JournalAmerican Journal of Reproductive Immunology
Volume67
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

Fingerprint

Chorioamnionitis
Pregnant Women
T-Lymphocytes
Biological Phenomena
Interleukin-15
Gene Ontology
T-Lymphocyte Subsets
Microarray Analysis
Placenta
Genes
Immunoglobulins
Signal Transduction
Leukocytes
Cell Death
Mothers
RNA
Membranes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Xu, Yi ; Tarquini, Federica ; Romero, Roberto ; Kim, Chong Jai ; Tarca, Adi L. ; Bhatti, Gaurav ; Lee, Joonho ; Sundell, I. Birgitta ; Mittal, Pooja ; Kusanovic, Juan Pedro ; Hassan, Sonia S. ; Kim, Jung Sun. / Peripheral CD300a+CD8+ T Lymphocytes with a Distinct Cytotoxic Molecular Signature Increase in Pregnant Women with Chronic Chorioamnionitis. In: American Journal of Reproductive Immunology. 2012 ; Vol. 67, No. 3. pp. 184-197.
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title = "Peripheral CD300a+CD8+ T Lymphocytes with a Distinct Cytotoxic Molecular Signature Increase in Pregnant Women with Chronic Chorioamnionitis",
abstract = "Problem CD300a is an immunomodulatory molecule of the immunoglobulin receptor superfamily expressed in the leukocytes of myeloid and lymphoid lineages. However, its biological function on CD8+ T lymphocytes remains largely unknown. This study was conducted to assess the biological significance of CD300a expression in T lymphocytes and to determine whether its expression in peripheral T lymphocytes changes in pregnant women presenting with antifetal rejection. Methods of Study Microarray analysis was performed using total RNA isolated from peripheral CD300a+ and CD300a- T lymphocytes. Flow cytometric analysis of the peripheral blood samples of pregnant women and pathologic examination of the placentas were conducted. Results A large number of genes (N=1245) were differentially expressed between CD300a- and CD300a+ subsets of CD8+ T lymphocytes, which included CCR7, CD244, CX3CR1, GLNY, GZMB, GZMK, IL15, ITGB1, KLRG1, PRF1, and SLAMF7. Gene ontology analysis of differentially expressed genes demonstrated enrichment of biological processes such as immune response, cell death, and signal transduction. CD300a expression in CD8+ T lymphocytes was coupled to a more cytotoxic molecular signature. Of note, the proportion of CD300a+CD8+ T lymphocytes increased in pregnant women with chronic chorioamnionitis (antifetal rejection of the chorioamniotic membranes; P<0.05). Conclusion The findings of this study strongly suggest an increase in systemic T-lymphocyte-mediated cytotoxicity in pregnant women with chronic chorioamnionitis as a manifestation of maternal antifetal rejection.",
author = "Yi Xu and Federica Tarquini and Roberto Romero and Kim, {Chong Jai} and Tarca, {Adi L.} and Gaurav Bhatti and Joonho Lee and Sundell, {I. Birgitta} and Pooja Mittal and Kusanovic, {Juan Pedro} and Hassan, {Sonia S.} and Kim, {Jung Sun}",
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language = "English",
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Xu, Y, Tarquini, F, Romero, R, Kim, CJ, Tarca, AL, Bhatti, G, Lee, J, Sundell, IB, Mittal, P, Kusanovic, JP, Hassan, SS & Kim, JS 2012, 'Peripheral CD300a+CD8+ T Lymphocytes with a Distinct Cytotoxic Molecular Signature Increase in Pregnant Women with Chronic Chorioamnionitis', American Journal of Reproductive Immunology, vol. 67, no. 3, pp. 184-197. https://doi.org/10.1111/j.1600-0897.2011.01088.x

Peripheral CD300a+CD8+ T Lymphocytes with a Distinct Cytotoxic Molecular Signature Increase in Pregnant Women with Chronic Chorioamnionitis. / Xu, Yi; Tarquini, Federica; Romero, Roberto; Kim, Chong Jai; Tarca, Adi L.; Bhatti, Gaurav; Lee, Joonho; Sundell, I. Birgitta; Mittal, Pooja; Kusanovic, Juan Pedro; Hassan, Sonia S.; Kim, Jung Sun.

In: American Journal of Reproductive Immunology, Vol. 67, No. 3, 01.03.2012, p. 184-197.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Peripheral CD300a+CD8+ T Lymphocytes with a Distinct Cytotoxic Molecular Signature Increase in Pregnant Women with Chronic Chorioamnionitis

AU - Xu, Yi

AU - Tarquini, Federica

AU - Romero, Roberto

AU - Kim, Chong Jai

AU - Tarca, Adi L.

AU - Bhatti, Gaurav

AU - Lee, Joonho

AU - Sundell, I. Birgitta

AU - Mittal, Pooja

AU - Kusanovic, Juan Pedro

AU - Hassan, Sonia S.

AU - Kim, Jung Sun

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Problem CD300a is an immunomodulatory molecule of the immunoglobulin receptor superfamily expressed in the leukocytes of myeloid and lymphoid lineages. However, its biological function on CD8+ T lymphocytes remains largely unknown. This study was conducted to assess the biological significance of CD300a expression in T lymphocytes and to determine whether its expression in peripheral T lymphocytes changes in pregnant women presenting with antifetal rejection. Methods of Study Microarray analysis was performed using total RNA isolated from peripheral CD300a+ and CD300a- T lymphocytes. Flow cytometric analysis of the peripheral blood samples of pregnant women and pathologic examination of the placentas were conducted. Results A large number of genes (N=1245) were differentially expressed between CD300a- and CD300a+ subsets of CD8+ T lymphocytes, which included CCR7, CD244, CX3CR1, GLNY, GZMB, GZMK, IL15, ITGB1, KLRG1, PRF1, and SLAMF7. Gene ontology analysis of differentially expressed genes demonstrated enrichment of biological processes such as immune response, cell death, and signal transduction. CD300a expression in CD8+ T lymphocytes was coupled to a more cytotoxic molecular signature. Of note, the proportion of CD300a+CD8+ T lymphocytes increased in pregnant women with chronic chorioamnionitis (antifetal rejection of the chorioamniotic membranes; P<0.05). Conclusion The findings of this study strongly suggest an increase in systemic T-lymphocyte-mediated cytotoxicity in pregnant women with chronic chorioamnionitis as a manifestation of maternal antifetal rejection.

AB - Problem CD300a is an immunomodulatory molecule of the immunoglobulin receptor superfamily expressed in the leukocytes of myeloid and lymphoid lineages. However, its biological function on CD8+ T lymphocytes remains largely unknown. This study was conducted to assess the biological significance of CD300a expression in T lymphocytes and to determine whether its expression in peripheral T lymphocytes changes in pregnant women presenting with antifetal rejection. Methods of Study Microarray analysis was performed using total RNA isolated from peripheral CD300a+ and CD300a- T lymphocytes. Flow cytometric analysis of the peripheral blood samples of pregnant women and pathologic examination of the placentas were conducted. Results A large number of genes (N=1245) were differentially expressed between CD300a- and CD300a+ subsets of CD8+ T lymphocytes, which included CCR7, CD244, CX3CR1, GLNY, GZMB, GZMK, IL15, ITGB1, KLRG1, PRF1, and SLAMF7. Gene ontology analysis of differentially expressed genes demonstrated enrichment of biological processes such as immune response, cell death, and signal transduction. CD300a expression in CD8+ T lymphocytes was coupled to a more cytotoxic molecular signature. Of note, the proportion of CD300a+CD8+ T lymphocytes increased in pregnant women with chronic chorioamnionitis (antifetal rejection of the chorioamniotic membranes; P<0.05). Conclusion The findings of this study strongly suggest an increase in systemic T-lymphocyte-mediated cytotoxicity in pregnant women with chronic chorioamnionitis as a manifestation of maternal antifetal rejection.

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