Perturbations of lipids and oxidized phospholipids in lipoproteins of patients with postmenopausal osteoporosis evaluated by asymmetrical flow field-flow fractionation and nanoflow UHPLC–ESI–MS/MS

Kang Geun Lee, Gwang Bin Lee, Joon Seon Yang, Myeong Hee Moon

Research output: Contribution to journalArticle

Abstract

Osteoporosis, a degenerative bone disease characterized by reduced bone mass and high risk of fragility, is associated with the alteration of circulating lipids, especially oxidized phospholipids (Ox-PLs). This study evaluated the lipidomic changes in lipoproteins of patients with postmenopausal osteoporosis (PMOp) vs. postmenopausal healthy controls. High-density lipoproteins (HDL) and low-density lipoproteins (LDL) from plasma samples were size-sorted by asymmetrical flow field-flow fractionation (AF4). Lipids from each lipoprotein were analyzed by nanoflow ultrahigh performance liquid chromatography–electrospray ionization–tandem mass spectrometry (nUHPLC–ESI–MS/MS). A significant difference was observed in a subset of lipids, most of which were increased in patients with PMOp, when compared to control. Phosphatidylethanolamine plasmalogen, which plays an antioxidative role, was increased in both lipoproteins (P-16:0/20:4, P-18:0/20:4, and P-18:1/20:4) lysophosphatidic acid 16:0, and six phosphatidylcholines were largely increased in HDL, but triacylglycerols (50:4 and 54:6) and overall ceramide levels were significantly increased only in LDL of patients with PMOp. Further investigation of 33 Ox-PLs showed significant lipid oxidation in PLs with highly unsaturated acyl chains, which were decreased in LDL of patients with PMOp. The present study demonstrated that AF4 with nUHPLC–ESI–MS/MS can be utilized to systematically profile Ox-PLs in the LDL of patients with PMOp.

Original languageEnglish
Article number46
JournalAntioxidants
Volume9
Issue number1
DOIs
Publication statusPublished - 2020 Jan

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Perturbations of lipids and oxidized phospholipids in lipoproteins of patients with postmenopausal osteoporosis evaluated by asymmetrical flow field-flow fractionation and nanoflow UHPLC–ESI–MS/MS'. Together they form a unique fingerprint.

  • Cite this