Pescadillo plays an essential role in plant cell growth and survival by modulating ribosome biogenesis

Hui Kyung Cho, Chang Sook Ahn, Ho Seok Lee, Ju Kon Kim, Hyun Sook Pai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Pescadillo (PES) is involved in diverse cellular processes such as embryonic development, ribosomal biogenesis, cell proliferation, and gene transcription in yeast and metazoans. In this study, we characterized cellular functions of plant PES in Nicotiana benthamiana, Arabidopsis, and tobacco BY-2 cells. A GFP fusion protein of PES is predominantly localized in the nucleolus, where its localization requires the N-terminal domain of PES. Silencing of plant PES led to growth arrest and acute cell death. PES interacts with plant homologs of BOP1 and WDR12 in the nucleolus, which are also nucleolar proteins involved in ribosome biogenesis of yeast and mammals. PES, BOP1, and WDR12 cofractionated with ribosome subunits. Depletion of any of these proteins led to defective biogenesis of the 60S ribosome large subunits and disruption of nucleolar morphology. PES-deficient plant cells also exhibited delayed maturation of 25S ribosomal RNA and suppressed global translation. During mitosis in tobacco BY-2 cells, PES is associated with the mitotic microtubules, including spindles and phragmoplasts, and PES deficiency disrupted spindle organization and chromosome arrangement. Collectively, these results suggest that plant PES has an essential role in cell growth and survival through its regulation of ribosome biogenesis and mitotic progression.

Original languageEnglish
Pages (from-to)393-405
Number of pages13
JournalPlant Journal
Volume76
Issue number3
DOIs
Publication statusPublished - 2013 Nov 1

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Plant Cells
ribosomes
Ribosomes
cell viability
cell growth
Cell Survival
Tobacco
Growth
cell nucleolus
Eukaryotic Large Ribosome Subunits
Yeasts
Ribosome Subunits
tobacco
yeasts
Nuclear Proteins
Arabidopsis
Mitosis
Microtubules
proteins
Nicotiana benthamiana

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Cho, Hui Kyung ; Ahn, Chang Sook ; Lee, Ho Seok ; Kim, Ju Kon ; Pai, Hyun Sook. / Pescadillo plays an essential role in plant cell growth and survival by modulating ribosome biogenesis. In: Plant Journal. 2013 ; Vol. 76, No. 3. pp. 393-405.
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Pescadillo plays an essential role in plant cell growth and survival by modulating ribosome biogenesis. / Cho, Hui Kyung; Ahn, Chang Sook; Lee, Ho Seok; Kim, Ju Kon; Pai, Hyun Sook.

In: Plant Journal, Vol. 76, No. 3, 01.11.2013, p. 393-405.

Research output: Contribution to journalArticle

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