PGC-enriched miRNAs control germ cell development

Jinhyuk Bhin, Hoe Su Jeong, Jong Soo Kim, Jeong Oh Shin, Ki Sung Hong, Han Sung Jung, Changhoon Kim, Daehee Hwang, Kye Seong Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unc lear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, - 106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysi sin PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through whic h these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.

Original languageEnglish
Pages (from-to)895-903
Number of pages9
JournalMolecules and cells
Volume38
Issue number10
DOIs
Publication statusPublished - 2015 Oct 7

Fingerprint

MicroRNAs
Germ Cells
Messenger RNA
Gonads
Microarray Analysis
Growth and Development
Northern Blotting
In Situ Hybridization
Testis

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Bhin, J., Jeong, H. S., Kim, J. S., Shin, J. O., Hong, K. S., Jung, H. S., ... Kim, K. S. (2015). PGC-enriched miRNAs control germ cell development. Molecules and cells, 38(10), 895-903. https://doi.org/10.14348/molcells.2015.0146
Bhin, Jinhyuk ; Jeong, Hoe Su ; Kim, Jong Soo ; Shin, Jeong Oh ; Hong, Ki Sung ; Jung, Han Sung ; Kim, Changhoon ; Hwang, Daehee ; Kim, Kye Seong. / PGC-enriched miRNAs control germ cell development. In: Molecules and cells. 2015 ; Vol. 38, No. 10. pp. 895-903.
@article{8d2ed3b7a75049979f35137f27aa006e,
title = "PGC-enriched miRNAs control germ cell development",
abstract = "Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unc lear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, - 106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysi sin PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through whic h these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.",
author = "Jinhyuk Bhin and Jeong, {Hoe Su} and Kim, {Jong Soo} and Shin, {Jeong Oh} and Hong, {Ki Sung} and Jung, {Han Sung} and Changhoon Kim and Daehee Hwang and Kim, {Kye Seong}",
year = "2015",
month = "10",
day = "7",
doi = "10.14348/molcells.2015.0146",
language = "English",
volume = "38",
pages = "895--903",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "10",

}

Bhin, J, Jeong, HS, Kim, JS, Shin, JO, Hong, KS, Jung, HS, Kim, C, Hwang, D & Kim, KS 2015, 'PGC-enriched miRNAs control germ cell development', Molecules and cells, vol. 38, no. 10, pp. 895-903. https://doi.org/10.14348/molcells.2015.0146

PGC-enriched miRNAs control germ cell development. / Bhin, Jinhyuk; Jeong, Hoe Su; Kim, Jong Soo; Shin, Jeong Oh; Hong, Ki Sung; Jung, Han Sung; Kim, Changhoon; Hwang, Daehee; Kim, Kye Seong.

In: Molecules and cells, Vol. 38, No. 10, 07.10.2015, p. 895-903.

Research output: Contribution to journalArticle

TY - JOUR

T1 - PGC-enriched miRNAs control germ cell development

AU - Bhin, Jinhyuk

AU - Jeong, Hoe Su

AU - Kim, Jong Soo

AU - Shin, Jeong Oh

AU - Hong, Ki Sung

AU - Jung, Han Sung

AU - Kim, Changhoon

AU - Hwang, Daehee

AU - Kim, Kye Seong

PY - 2015/10/7

Y1 - 2015/10/7

N2 - Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unc lear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, - 106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysi sin PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through whic h these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.

AB - Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unc lear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, - 106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysi sin PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through whic h these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.

UR - http://www.scopus.com/inward/record.url?scp=84950291931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84950291931&partnerID=8YFLogxK

U2 - 10.14348/molcells.2015.0146

DO - 10.14348/molcells.2015.0146

M3 - Article

C2 - 26442865

AN - SCOPUS:84950291931

VL - 38

SP - 895

EP - 903

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 10

ER -

Bhin J, Jeong HS, Kim JS, Shin JO, Hong KS, Jung HS et al. PGC-enriched miRNAs control germ cell development. Molecules and cells. 2015 Oct 7;38(10):895-903. https://doi.org/10.14348/molcells.2015.0146