Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.
|Title of host publication||Single Molecule Spectroscopy and Superresolution Imaging XII|
|Editors||Ingo Gregor, Felix Koberling, Zygmunt K. Gryczynski|
|Publication status||Published - 2019|
|Event||Single Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States|
Duration: 2019 Feb 2 → 2019 Feb 3
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Conference||Single Molecule Spectroscopy and Superresolution Imaging XII 2019|
|Period||19/2/2 → 19/2/3|
Bibliographical noteFunding Information:
This research has been funded by National Research Foundation of Korea (NRF-2013R1A1A2062448) through the Basic Science Research Program, Global Frontier Project (CAMM-2014M3A6B3063712) and the Ministry of Science and ICT of Korea, Technology Innovation Program (10062417), and the Ministry of Trade, Industry and Energy of Korea.
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging