Phasor plot analysis using low pass filter for high-speed FLIM

Dongeun Kim, Wonsang Hwang, Youngjae Won, Sucbei Moon, Sang Yoon Lee, Mingu Kang, Won Sub Han, Dug Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging XII
EditorsIngo Gregor, Felix Koberling, Zygmunt K. Gryczynski
PublisherSPIE
ISBN (Electronic)9781510624108
DOIs
Publication statusPublished - 2019 Jan 1
EventSingle Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States
Duration: 2019 Feb 22019 Feb 3

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10884
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging XII 2019
CountryUnited States
CitySan Francisco
Period19/2/219/2/3

Fingerprint

low pass filters
Low pass filters
plots
high speed
acquisition
Fluorescence
life (durability)
fluorescence
Optical Imaging
Frequency modulation
Time measurement
Photons
frequency modulation
Computer hardware
Microscopy
Microscopic examination
hardware
sampling
time measurement
Sampling

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Kim, D., Hwang, W., Won, Y., Moon, S., Lee, S. Y., Kang, M., ... Kim, D. Y. (2019). Phasor plot analysis using low pass filter for high-speed FLIM. In I. Gregor, F. Koberling, & Z. K. Gryczynski (Eds.), Single Molecule Spectroscopy and Superresolution Imaging XII [108840P] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884). SPIE. https://doi.org/10.1117/12.2509325
Kim, Dongeun ; Hwang, Wonsang ; Won, Youngjae ; Moon, Sucbei ; Lee, Sang Yoon ; Kang, Mingu ; Han, Won Sub ; Kim, Dug Young. / Phasor plot analysis using low pass filter for high-speed FLIM. Single Molecule Spectroscopy and Superresolution Imaging XII. editor / Ingo Gregor ; Felix Koberling ; Zygmunt K. Gryczynski. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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abstract = "Phasor plot analysis is one of the most powerful analysis technique in fluorescence lifetime imaging microscopy, especially for analysis of heterogeneous mixtures. Compared to frequency domain fluorescence lifetime measurement, time domain measurement offers information in various frequencies at once measurement, but needs high frequency sampling for stable signal acquisition, which requires a lot of memory in hardware and a long time for analysis, furthermore in TCSPC, acquisition time is extremely long due to low photon count rate. We suggest a new system with low pass filter, which leads to about 100 times faster measurement speed while maintaining precision and accuracy in usual modulation frequency.",
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Kim, D, Hwang, W, Won, Y, Moon, S, Lee, SY, Kang, M, Han, WS & Kim, DY 2019, Phasor plot analysis using low pass filter for high-speed FLIM. in I Gregor, F Koberling & ZK Gryczynski (eds), Single Molecule Spectroscopy and Superresolution Imaging XII., 108840P, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10884, SPIE, Single Molecule Spectroscopy and Superresolution Imaging XII 2019, San Francisco, United States, 19/2/2. https://doi.org/10.1117/12.2509325

Phasor plot analysis using low pass filter for high-speed FLIM. / Kim, Dongeun; Hwang, Wonsang; Won, Youngjae; Moon, Sucbei; Lee, Sang Yoon; Kang, Mingu; Han, Won Sub; Kim, Dug Young.

Single Molecule Spectroscopy and Superresolution Imaging XII. ed. / Ingo Gregor; Felix Koberling; Zygmunt K. Gryczynski. SPIE, 2019. 108840P (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10884).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Kim D, Hwang W, Won Y, Moon S, Lee SY, Kang M et al. Phasor plot analysis using low pass filter for high-speed FLIM. In Gregor I, Koberling F, Gryczynski ZK, editors, Single Molecule Spectroscopy and Superresolution Imaging XII. SPIE. 2019. 108840P. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2509325