Pheromone sensing regulates Caenorhabditis elegans lifespan and stress resistance via the deacetylase SIR-2.1

Andreas H. Ludewig, Yevgeniy Izrayelit, Donha Park, Rabia U. Malik, Anna Zimmermann, Parag Mahanti, Bennett W. Fox, Axel Bethke, Frank Doering, Donald L. Riddle, Frank C. Schroeder

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Lifespan in Caenorhabditis elegans, Drosophila, and mice is regulated by conserved signaling networks, including the insulin/insulinlike growth factor 1 (IGF-1) signaling cascade and pathways depending on sirtuins, a family of NAD+-dependent deacetylases. Small molecules such as resveratrol are of great interest because they increase lifespan in many species in a sirtuin-dependent manner. However, no endogenous small molecules that regulate lifespan via sirtuins have been identified, and the mechanisms underlying sirtuin-dependent longevity are not well understood. Here, we show that in C. elegans, two endogenously produced small molecules, the dauer-inducing ascarosides ascr#2 and ascr#3, regulate lifespan and stress resistance through chemosensory pathways and the sirtuin SIR-2.1. Ascarosides extend adult lifespan and stress resistance without reducing fecundity or feeding rate, and these effects are reduced or abolished when nutrients are restricted. We found that ascaroside-mediated longevity is fully abolished by loss of SIR-2.1 and that the effect of ascr#2 requires expression of the G protein-coupled receptor DAF-37 in specific chemosensory neurons. In contrast to many other lifespan-modulating factors, ascarosidemediated lifespan increases do not require insulin signaling via the FOXOhomologDAF-16 or the insulin/IGF-1-receptor homologDAF-2. Our study demonstrates that C. elegans produces specific small molecules to control adult lifespan in a sirtuin-dependent manner, supporting the hypothesis that endogenous regulation ofmetazoan lifespan functions, in part, via sirtuins. These findings strengthen the link between chemosensory inputs and conserved mechanisms of lifespan regulation in metazoans and suggest a model for communal lifespan regulation in C. elegans.

Original languageEnglish
Pages (from-to)5522-5527
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number14
DOIs
Publication statusPublished - 2013 Apr 2

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Pheromones
Sirtuins
Caenorhabditis elegans
Insulin
Growth Factor Receptors
G-Protein-Coupled Receptors
NAD
Drosophila
Fertility
Intercellular Signaling Peptides and Proteins
Neurons
Food

All Science Journal Classification (ASJC) codes

  • General

Cite this

Ludewig, Andreas H. ; Izrayelit, Yevgeniy ; Park, Donha ; Malik, Rabia U. ; Zimmermann, Anna ; Mahanti, Parag ; Fox, Bennett W. ; Bethke, Axel ; Doering, Frank ; Riddle, Donald L. ; Schroeder, Frank C. / Pheromone sensing regulates Caenorhabditis elegans lifespan and stress resistance via the deacetylase SIR-2.1. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 14. pp. 5522-5527.
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abstract = "Lifespan in Caenorhabditis elegans, Drosophila, and mice is regulated by conserved signaling networks, including the insulin/insulinlike growth factor 1 (IGF-1) signaling cascade and pathways depending on sirtuins, a family of NAD+-dependent deacetylases. Small molecules such as resveratrol are of great interest because they increase lifespan in many species in a sirtuin-dependent manner. However, no endogenous small molecules that regulate lifespan via sirtuins have been identified, and the mechanisms underlying sirtuin-dependent longevity are not well understood. Here, we show that in C. elegans, two endogenously produced small molecules, the dauer-inducing ascarosides ascr#2 and ascr#3, regulate lifespan and stress resistance through chemosensory pathways and the sirtuin SIR-2.1. Ascarosides extend adult lifespan and stress resistance without reducing fecundity or feeding rate, and these effects are reduced or abolished when nutrients are restricted. We found that ascaroside-mediated longevity is fully abolished by loss of SIR-2.1 and that the effect of ascr#2 requires expression of the G protein-coupled receptor DAF-37 in specific chemosensory neurons. In contrast to many other lifespan-modulating factors, ascarosidemediated lifespan increases do not require insulin signaling via the FOXOhomologDAF-16 or the insulin/IGF-1-receptor homologDAF-2. Our study demonstrates that C. elegans produces specific small molecules to control adult lifespan in a sirtuin-dependent manner, supporting the hypothesis that endogenous regulation ofmetazoan lifespan functions, in part, via sirtuins. These findings strengthen the link between chemosensory inputs and conserved mechanisms of lifespan regulation in metazoans and suggest a model for communal lifespan regulation in C. elegans.",
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Ludewig, AH, Izrayelit, Y, Park, D, Malik, RU, Zimmermann, A, Mahanti, P, Fox, BW, Bethke, A, Doering, F, Riddle, DL & Schroeder, FC 2013, 'Pheromone sensing regulates Caenorhabditis elegans lifespan and stress resistance via the deacetylase SIR-2.1', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 14, pp. 5522-5527. https://doi.org/10.1073/pnas.1214467110

Pheromone sensing regulates Caenorhabditis elegans lifespan and stress resistance via the deacetylase SIR-2.1. / Ludewig, Andreas H.; Izrayelit, Yevgeniy; Park, Donha; Malik, Rabia U.; Zimmermann, Anna; Mahanti, Parag; Fox, Bennett W.; Bethke, Axel; Doering, Frank; Riddle, Donald L.; Schroeder, Frank C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 14, 02.04.2013, p. 5522-5527.

Research output: Contribution to journalArticle

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T1 - Pheromone sensing regulates Caenorhabditis elegans lifespan and stress resistance via the deacetylase SIR-2.1

AU - Ludewig, Andreas H.

AU - Izrayelit, Yevgeniy

AU - Park, Donha

AU - Malik, Rabia U.

AU - Zimmermann, Anna

AU - Mahanti, Parag

AU - Fox, Bennett W.

AU - Bethke, Axel

AU - Doering, Frank

AU - Riddle, Donald L.

AU - Schroeder, Frank C.

PY - 2013/4/2

Y1 - 2013/4/2

N2 - Lifespan in Caenorhabditis elegans, Drosophila, and mice is regulated by conserved signaling networks, including the insulin/insulinlike growth factor 1 (IGF-1) signaling cascade and pathways depending on sirtuins, a family of NAD+-dependent deacetylases. Small molecules such as resveratrol are of great interest because they increase lifespan in many species in a sirtuin-dependent manner. However, no endogenous small molecules that regulate lifespan via sirtuins have been identified, and the mechanisms underlying sirtuin-dependent longevity are not well understood. Here, we show that in C. elegans, two endogenously produced small molecules, the dauer-inducing ascarosides ascr#2 and ascr#3, regulate lifespan and stress resistance through chemosensory pathways and the sirtuin SIR-2.1. Ascarosides extend adult lifespan and stress resistance without reducing fecundity or feeding rate, and these effects are reduced or abolished when nutrients are restricted. We found that ascaroside-mediated longevity is fully abolished by loss of SIR-2.1 and that the effect of ascr#2 requires expression of the G protein-coupled receptor DAF-37 in specific chemosensory neurons. In contrast to many other lifespan-modulating factors, ascarosidemediated lifespan increases do not require insulin signaling via the FOXOhomologDAF-16 or the insulin/IGF-1-receptor homologDAF-2. Our study demonstrates that C. elegans produces specific small molecules to control adult lifespan in a sirtuin-dependent manner, supporting the hypothesis that endogenous regulation ofmetazoan lifespan functions, in part, via sirtuins. These findings strengthen the link between chemosensory inputs and conserved mechanisms of lifespan regulation in metazoans and suggest a model for communal lifespan regulation in C. elegans.

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