Phorbol myristate acetate-induced Egr-1 expression is suppressed by phospholipase D isozymes in human glioma cells

Bong Hyun Ahn, Mi Hee Park, Young Han Lee, Do Sik Min

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Early growth response-1 (Egr-1) is involved in the regulation of cell growth. Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Suppression of PMA-induced Egr-1 was dependent on the expression level of PLD isozymes. Overexpression of catalytically inactive PLD, treatment with PA, and prevention of PA dephosphorylation by 1-propranolol significantly suppressed PMA-induced Egr-1 expression. PLD-induced suppression of Egr-1 was reversed by inhibition of phosphatidylinositol 3-kinase (PI3K). Taken together, these results suggest that elevated expression and activity of PLD attenuate PMA-induced Egr-1 expression via PI3K pathway.

Original languageEnglish
Pages (from-to)5940-5944
Number of pages5
JournalFEBS Letters
Volume581
Issue number30
DOIs
Publication statusPublished - 2007 Dec 22

Fingerprint

Phospholipase D
Tetradecanoylphorbol Acetate
Glioma
Isoenzymes
Growth
Phosphatidylinositol 3-Kinase
Cell growth
Propranolol
Carcinogens
Transcriptional Activation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ahn, Bong Hyun ; Park, Mi Hee ; Lee, Young Han ; Min, Do Sik. / Phorbol myristate acetate-induced Egr-1 expression is suppressed by phospholipase D isozymes in human glioma cells. In: FEBS Letters. 2007 ; Vol. 581, No. 30. pp. 5940-5944.
@article{738a7664e57b403f99a52587cf95cc59,
title = "Phorbol myristate acetate-induced Egr-1 expression is suppressed by phospholipase D isozymes in human glioma cells",
abstract = "Early growth response-1 (Egr-1) is involved in the regulation of cell growth. Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Suppression of PMA-induced Egr-1 was dependent on the expression level of PLD isozymes. Overexpression of catalytically inactive PLD, treatment with PA, and prevention of PA dephosphorylation by 1-propranolol significantly suppressed PMA-induced Egr-1 expression. PLD-induced suppression of Egr-1 was reversed by inhibition of phosphatidylinositol 3-kinase (PI3K). Taken together, these results suggest that elevated expression and activity of PLD attenuate PMA-induced Egr-1 expression via PI3K pathway.",
author = "Ahn, {Bong Hyun} and Park, {Mi Hee} and Lee, {Young Han} and Min, {Do Sik}",
year = "2007",
month = "12",
day = "22",
doi = "10.1016/j.febslet.2007.11.077",
language = "English",
volume = "581",
pages = "5940--5944",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "30",

}

Phorbol myristate acetate-induced Egr-1 expression is suppressed by phospholipase D isozymes in human glioma cells. / Ahn, Bong Hyun; Park, Mi Hee; Lee, Young Han; Min, Do Sik.

In: FEBS Letters, Vol. 581, No. 30, 22.12.2007, p. 5940-5944.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phorbol myristate acetate-induced Egr-1 expression is suppressed by phospholipase D isozymes in human glioma cells

AU - Ahn, Bong Hyun

AU - Park, Mi Hee

AU - Lee, Young Han

AU - Min, Do Sik

PY - 2007/12/22

Y1 - 2007/12/22

N2 - Early growth response-1 (Egr-1) is involved in the regulation of cell growth. Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Suppression of PMA-induced Egr-1 was dependent on the expression level of PLD isozymes. Overexpression of catalytically inactive PLD, treatment with PA, and prevention of PA dephosphorylation by 1-propranolol significantly suppressed PMA-induced Egr-1 expression. PLD-induced suppression of Egr-1 was reversed by inhibition of phosphatidylinositol 3-kinase (PI3K). Taken together, these results suggest that elevated expression and activity of PLD attenuate PMA-induced Egr-1 expression via PI3K pathway.

AB - Early growth response-1 (Egr-1) is involved in the regulation of cell growth. Here, we found that overexpression of phospholipase D (PLD) isozymes decreased tumor promoter phorbol myristate acetate (PMA)-induced Egr-1 expression and transactivation in glioma cells. Suppression of PMA-induced Egr-1 was dependent on the expression level of PLD isozymes. Overexpression of catalytically inactive PLD, treatment with PA, and prevention of PA dephosphorylation by 1-propranolol significantly suppressed PMA-induced Egr-1 expression. PLD-induced suppression of Egr-1 was reversed by inhibition of phosphatidylinositol 3-kinase (PI3K). Taken together, these results suggest that elevated expression and activity of PLD attenuate PMA-induced Egr-1 expression via PI3K pathway.

UR - http://www.scopus.com/inward/record.url?scp=37049028810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37049028810&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.11.077

DO - 10.1016/j.febslet.2007.11.077

M3 - Article

C2 - 18067864

AN - SCOPUS:37049028810

VL - 581

SP - 5940

EP - 5944

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 30

ER -