Phospholipase C-δ1 rescues intracellular Ca2+ overload in ischemic heart and hypoxic neonatal cardiomyocytes

Ki Chul Hwang, Soyeon Lim, Hyuck Moon Kwon, Yun Soo Bae, seokmin kang, Kwang Hoe Chung, Robert M. Graham, Sue Goo Rhee, Yangsoo Jang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ischemia and simulated ischemic conditions cause intracellular Ca 2+ overload in the myocardium. The relationship between ischemia injury and Ca2+ overload has not been fully characterized. The aim of the present study was to investigate the expression and characteristics of PLC isozymes in myocardial infarction-induced cardiac remodeling and heart failure. In normal rat heart tissue, PLC-δ1 (about 44 ng/mg of heart tissue) was most abundant isozymes compared to PLC-γ1 (6.8 ng/mg) and PLC-β1 (0.4 ng/mg). In ischemic heart and hypoxic neonatal cardiomyocytes, PLC-δ1, but not PLC-β1 and PLC-γ1, was selectively degraded, a response that could be inhibited by the calpain inhibitor, calpastatin, and by the caspase inhibitor, zVAD-fmk. Overexpression of the PLC-δ1 in hypoxic neonatal cardiomyocytes rescued intracellular Ca2+ overload by ischemic conditions. In the border zone and scar region of infarcted myocardium, and in hypoxic neonatal cardiomyocytes, the selective degradation of PLC-δ1 by the calcium sensitive proteases may play important roles in intracellular Ca2+ regulations under the ischemic conditions. It is suggested that PLC isozyme-changes may contribute to the alterations in calcium homeostasis in myocardial ischemia.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume91
Issue number3
DOIs
Publication statusPublished - 2004 Jul 1

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Type C Phospholipases
Programmable logic controllers
Cardiac Myocytes
Isoenzymes
Myocardium
Ischemia
Heart Failure
Calcium
Caspase Inhibitors
Cicatrix
Myocardial Ischemia
Homeostasis
Peptide Hydrolases
Myocardial Infarction
Wounds and Injuries
Tissue
Rats
Degradation

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Hwang, Ki Chul ; Lim, Soyeon ; Kwon, Hyuck Moon ; Bae, Yun Soo ; kang, seokmin ; Chung, Kwang Hoe ; Graham, Robert M. ; Rhee, Sue Goo ; Jang, Yangsoo. / Phospholipase C-δ1 rescues intracellular Ca2+ overload in ischemic heart and hypoxic neonatal cardiomyocytes. In: Journal of Steroid Biochemistry and Molecular Biology. 2004 ; Vol. 91, No. 3. pp. 131-138.
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Phospholipase C-δ1 rescues intracellular Ca2+ overload in ischemic heart and hypoxic neonatal cardiomyocytes. / Hwang, Ki Chul; Lim, Soyeon; Kwon, Hyuck Moon; Bae, Yun Soo; kang, seokmin; Chung, Kwang Hoe; Graham, Robert M.; Rhee, Sue Goo; Jang, Yangsoo.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 91, No. 3, 01.07.2004, p. 131-138.

Research output: Contribution to journalArticle

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