Phospholipase C isozymes selectively couple to specific neurotransmitter receptors

Daesoo Kim, Ki Sun Jun, Seong Beom Lee, Nae Gyu Kang, Do Sik Min, Young Hoon Kim, Sung Ho Ryu, Pann Ghill Suh, Hee Sup Shin

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Abstract

A variety of extracellular signals are transduced across the cell membrane by the enzyme phosphoinositide-specific phospholipase C-β (PLC-β) coupled with guanine-nucleotide-binding G proteins. There are four isoenzymes of PLC-β, β1-β4, but their functions in vivo are not known. Here we investigate the role of PLC-β1 and PLC-β4 in the brain by generating null mutations in mice: we found that PLCβ1(-/-) mice developed epilepsy and PLCβ4(-/-) mice showed ataxia. We determined the molecular basis of these phenotypes and show that PLC-β is involved in signal transduction in the cerebral cortex and hippocampus by coupling pre-dominantly to the muscarinic acetylcholine receptor, whereas PLC-β4 works through the metabotropic glutamate receptor in the cerebellum, illustrating how PLG-β isoenzymes are used to generate different functions in the brain.

Original languageEnglish
Pages (from-to)290-293
Number of pages4
JournalNature
Volume389
Issue number6648
DOIs
Publication statusPublished - 1997 Oct 7

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Cite this

Kim, D., Jun, K. S., Lee, S. B., Kang, N. G., Min, D. S., Kim, Y. H., Ryu, S. H., Suh, P. G., & Shin, H. S. (1997). Phospholipase C isozymes selectively couple to specific neurotransmitter receptors. Nature, 389(6648), 290-293. https://doi.org/10.1038/38508