Phospholipase D2 activity suppresses hydrogen peroxide-induced apoptosis in PC12 cells

Sang Do Lee, Byoung Dae Lee, Jung Min Han, Jae Ho Kim, Yong Kim, Pann Ghill Suh, Sung Ho Ryu

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Phospholipase D (PLD) plays an important role as an effector in the membrane lipid-mediated signal transduction. However, the precise physiological functions of PLD are not yet well understood. In this study, we examined the role of PLD activity in hydrogen peroxide (H2O2)-induced apoptosis in rat pheochromocytoma (PC12) cells. Treatment of PC12 cells with H2O2 resulted in induction of apoptosis in these cells, which is accompanied by the activation of PLD. This H2O2-induced apoptosis was enhanced remarkably when phosphatidic acid production by PLD was selectively inhibited by pre-treating the PC12 cells with 1-butanol. Expression of PLD2, but not of PLD1, correlated with increased H2O2-induced PLD activity in a concentration- and time-dependent manner. Concomitant with PLD activation, the PLD2 activity suppressed H2O2-induced apoptosis in PC12 cells. Expression of PLD2 lipase-inactive mutant (K758R) had no effect on either PLD activity or apoptosis. PLD2 activity also suppressed H2O2-induced cleavage and activation of caspase-3. Taken together, the results suggest that PLD2 activity is specifically up-regulated by H2O2 in PC12 cells and that it plays a suppressive role in H2O2-induced apoptosis.

Original languageEnglish
Pages (from-to)1053-1059
Number of pages7
JournalJournal of Neurochemistry
Volume75
Issue number3
DOIs
Publication statusPublished - 2000 Sep 11

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Phospholipase D
PC12 Cells
Hydrogen Peroxide
Apoptosis
Chemical activation
Signal transduction
Phosphatidic Acids
1-Butanol
phospholipase D2
Pheochromocytoma
Membrane Lipids
Lipase
Caspase 3
Rats
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Lee, Sang Do ; Lee, Byoung Dae ; Han, Jung Min ; Kim, Jae Ho ; Kim, Yong ; Suh, Pann Ghill ; Ryu, Sung Ho. / Phospholipase D2 activity suppresses hydrogen peroxide-induced apoptosis in PC12 cells. In: Journal of Neurochemistry. 2000 ; Vol. 75, No. 3. pp. 1053-1059.
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Phospholipase D2 activity suppresses hydrogen peroxide-induced apoptosis in PC12 cells. / Lee, Sang Do; Lee, Byoung Dae; Han, Jung Min; Kim, Jae Ho; Kim, Yong; Suh, Pann Ghill; Ryu, Sung Ho.

In: Journal of Neurochemistry, Vol. 75, No. 3, 11.09.2000, p. 1053-1059.

Research output: Contribution to journalArticle

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AU - Lee, Sang Do

AU - Lee, Byoung Dae

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