Phosphorylation-dependent regulation of phospholipase D2 by protein kinase Cδ in rat pheochromocytoma PC12 cells

Jung Min Han, Jae Ho Kim, Byoung Dae Lee, Sang Do Lee, Yong Kim, Yon Woo Jung, Sukmook Lee, Wonhwa Cho, Motoi Ohba, Toshio Kuroki, Pann Ghill Suh, Sung Ho Ryu

Research output: Contribution to journalArticle

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Abstract

Many studies have shown that protein kinase C (PKC) is an important physiological regulator of phospholipase D (PLD). However, the role of PKC in agonist-induced PLD activation has been mainly investigated with a focus on the PLD1, which is one of the two PLD isoenzymes (PLD1 and PLD2) cloned to date. Since the expression of PLD2 significantly enhanced phorbol 12-myristate 13-acetate (PMA)- or bradykinin-induced PLD activity in rat pheochromocytoma PC12 cells, we investigated the regulatory mechanism of PLD2 in PC12 cells. Two different PKC inhibitors, GF109203X and Ro-31-8220, completely blocked PMA-induced PLD2 activation. In addition, specific inhibition of PKCδ by rottlerin prevented PLD2 activation in PMA-stimulated PC12 cells. Concomitant with PLD2 activation, PLD2 became phosphorylated upon PMA or bradykinin treatment of PC12 cells. Moreover, rottlerin blocked PMA- or bradykinin-induced PLD2 phosphorylation in PC12 cells. Expression of a kinase-deficient mutant of PKCδ using adenovirus-mediated gene transfer inhibited the phosphorylation and activation of PLD2 induced by PMA in PC12 cells, suggesting the phosphorylation-dependent regulation of PLD2 mediated by PKCδ kinase activity in PC12 cells. PKCδ co-immunoprecipitated with PLD2 from PC 12 cell extracts, and associated with PLD2 in vitro in a PMA. dependent manner. Phospho-PLD2 immunoprecipitated from PMA-treated PC12 cells and PLD2 phosphorylated in vitro by PKCδ were resolved by two-dimensional phosphopeptide mapping and compared. At least seven phosphopeptides co-migrated, indicating the direct phosphorylation of PLD2 by PKCδ inside the cells. Immunocytochemical studies of PC12 cells revealed that after treatment with PMA, PKCδ was translocated from the cytosol to the plasma membrane where PLD2 is mainly localized. These results suggest that PKCδ-dependent direct phosphorylation plays an important role in the regulation of PLD2 activity in PC12 cells.

Original languageEnglish
Pages (from-to)8290-8297
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number10
DOIs
Publication statusPublished - 2002 Mar 8

Fingerprint

Phosphorylation
PC12 Cells
Pheochromocytoma
Protein Kinase C
Rats
Acetates
Phospholipase D
Chemical activation
Bradykinin
Phosphopeptides
Acetate Kinase
phospholipase D2
Gene transfer
phorbol-12-myristate
Protein C Inhibitor
Mutant Proteins
Cell membranes
Protein Kinase Inhibitors
Cell Extracts
Adenoviridae

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Han, Jung Min ; Kim, Jae Ho ; Lee, Byoung Dae ; Lee, Sang Do ; Kim, Yong ; Jung, Yon Woo ; Lee, Sukmook ; Cho, Wonhwa ; Ohba, Motoi ; Kuroki, Toshio ; Suh, Pann Ghill ; Ryu, Sung Ho. / Phosphorylation-dependent regulation of phospholipase D2 by protein kinase Cδ in rat pheochromocytoma PC12 cells. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 10. pp. 8290-8297.
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abstract = "Many studies have shown that protein kinase C (PKC) is an important physiological regulator of phospholipase D (PLD). However, the role of PKC in agonist-induced PLD activation has been mainly investigated with a focus on the PLD1, which is one of the two PLD isoenzymes (PLD1 and PLD2) cloned to date. Since the expression of PLD2 significantly enhanced phorbol 12-myristate 13-acetate (PMA)- or bradykinin-induced PLD activity in rat pheochromocytoma PC12 cells, we investigated the regulatory mechanism of PLD2 in PC12 cells. Two different PKC inhibitors, GF109203X and Ro-31-8220, completely blocked PMA-induced PLD2 activation. In addition, specific inhibition of PKCδ by rottlerin prevented PLD2 activation in PMA-stimulated PC12 cells. Concomitant with PLD2 activation, PLD2 became phosphorylated upon PMA or bradykinin treatment of PC12 cells. Moreover, rottlerin blocked PMA- or bradykinin-induced PLD2 phosphorylation in PC12 cells. Expression of a kinase-deficient mutant of PKCδ using adenovirus-mediated gene transfer inhibited the phosphorylation and activation of PLD2 induced by PMA in PC12 cells, suggesting the phosphorylation-dependent regulation of PLD2 mediated by PKCδ kinase activity in PC12 cells. PKCδ co-immunoprecipitated with PLD2 from PC 12 cell extracts, and associated with PLD2 in vitro in a PMA. dependent manner. Phospho-PLD2 immunoprecipitated from PMA-treated PC12 cells and PLD2 phosphorylated in vitro by PKCδ were resolved by two-dimensional phosphopeptide mapping and compared. At least seven phosphopeptides co-migrated, indicating the direct phosphorylation of PLD2 by PKCδ inside the cells. Immunocytochemical studies of PC12 cells revealed that after treatment with PMA, PKCδ was translocated from the cytosol to the plasma membrane where PLD2 is mainly localized. These results suggest that PKCδ-dependent direct phosphorylation plays an important role in the regulation of PLD2 activity in PC12 cells.",
author = "Han, {Jung Min} and Kim, {Jae Ho} and Lee, {Byoung Dae} and Lee, {Sang Do} and Yong Kim and Jung, {Yon Woo} and Sukmook Lee and Wonhwa Cho and Motoi Ohba and Toshio Kuroki and Suh, {Pann Ghill} and Ryu, {Sung Ho}",
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Han, JM, Kim, JH, Lee, BD, Lee, SD, Kim, Y, Jung, YW, Lee, S, Cho, W, Ohba, M, Kuroki, T, Suh, PG & Ryu, SH 2002, 'Phosphorylation-dependent regulation of phospholipase D2 by protein kinase Cδ in rat pheochromocytoma PC12 cells', Journal of Biological Chemistry, vol. 277, no. 10, pp. 8290-8297. https://doi.org/10.1074/jbc.M108343200

Phosphorylation-dependent regulation of phospholipase D2 by protein kinase Cδ in rat pheochromocytoma PC12 cells. / Han, Jung Min; Kim, Jae Ho; Lee, Byoung Dae; Lee, Sang Do; Kim, Yong; Jung, Yon Woo; Lee, Sukmook; Cho, Wonhwa; Ohba, Motoi; Kuroki, Toshio; Suh, Pann Ghill; Ryu, Sung Ho.

In: Journal of Biological Chemistry, Vol. 277, No. 10, 08.03.2002, p. 8290-8297.

Research output: Contribution to journalArticle

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AU - Han, Jung Min

AU - Kim, Jae Ho

AU - Lee, Byoung Dae

AU - Lee, Sang Do

AU - Kim, Yong

AU - Jung, Yon Woo

AU - Lee, Sukmook

AU - Cho, Wonhwa

AU - Ohba, Motoi

AU - Kuroki, Toshio

AU - Suh, Pann Ghill

AU - Ryu, Sung Ho

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N2 - Many studies have shown that protein kinase C (PKC) is an important physiological regulator of phospholipase D (PLD). However, the role of PKC in agonist-induced PLD activation has been mainly investigated with a focus on the PLD1, which is one of the two PLD isoenzymes (PLD1 and PLD2) cloned to date. Since the expression of PLD2 significantly enhanced phorbol 12-myristate 13-acetate (PMA)- or bradykinin-induced PLD activity in rat pheochromocytoma PC12 cells, we investigated the regulatory mechanism of PLD2 in PC12 cells. Two different PKC inhibitors, GF109203X and Ro-31-8220, completely blocked PMA-induced PLD2 activation. In addition, specific inhibition of PKCδ by rottlerin prevented PLD2 activation in PMA-stimulated PC12 cells. Concomitant with PLD2 activation, PLD2 became phosphorylated upon PMA or bradykinin treatment of PC12 cells. Moreover, rottlerin blocked PMA- or bradykinin-induced PLD2 phosphorylation in PC12 cells. Expression of a kinase-deficient mutant of PKCδ using adenovirus-mediated gene transfer inhibited the phosphorylation and activation of PLD2 induced by PMA in PC12 cells, suggesting the phosphorylation-dependent regulation of PLD2 mediated by PKCδ kinase activity in PC12 cells. PKCδ co-immunoprecipitated with PLD2 from PC 12 cell extracts, and associated with PLD2 in vitro in a PMA. dependent manner. Phospho-PLD2 immunoprecipitated from PMA-treated PC12 cells and PLD2 phosphorylated in vitro by PKCδ were resolved by two-dimensional phosphopeptide mapping and compared. At least seven phosphopeptides co-migrated, indicating the direct phosphorylation of PLD2 by PKCδ inside the cells. Immunocytochemical studies of PC12 cells revealed that after treatment with PMA, PKCδ was translocated from the cytosol to the plasma membrane where PLD2 is mainly localized. These results suggest that PKCδ-dependent direct phosphorylation plays an important role in the regulation of PLD2 activity in PC12 cells.

AB - Many studies have shown that protein kinase C (PKC) is an important physiological regulator of phospholipase D (PLD). However, the role of PKC in agonist-induced PLD activation has been mainly investigated with a focus on the PLD1, which is one of the two PLD isoenzymes (PLD1 and PLD2) cloned to date. Since the expression of PLD2 significantly enhanced phorbol 12-myristate 13-acetate (PMA)- or bradykinin-induced PLD activity in rat pheochromocytoma PC12 cells, we investigated the regulatory mechanism of PLD2 in PC12 cells. Two different PKC inhibitors, GF109203X and Ro-31-8220, completely blocked PMA-induced PLD2 activation. In addition, specific inhibition of PKCδ by rottlerin prevented PLD2 activation in PMA-stimulated PC12 cells. Concomitant with PLD2 activation, PLD2 became phosphorylated upon PMA or bradykinin treatment of PC12 cells. Moreover, rottlerin blocked PMA- or bradykinin-induced PLD2 phosphorylation in PC12 cells. Expression of a kinase-deficient mutant of PKCδ using adenovirus-mediated gene transfer inhibited the phosphorylation and activation of PLD2 induced by PMA in PC12 cells, suggesting the phosphorylation-dependent regulation of PLD2 mediated by PKCδ kinase activity in PC12 cells. PKCδ co-immunoprecipitated with PLD2 from PC 12 cell extracts, and associated with PLD2 in vitro in a PMA. dependent manner. Phospho-PLD2 immunoprecipitated from PMA-treated PC12 cells and PLD2 phosphorylated in vitro by PKCδ were resolved by two-dimensional phosphopeptide mapping and compared. At least seven phosphopeptides co-migrated, indicating the direct phosphorylation of PLD2 by PKCδ inside the cells. Immunocytochemical studies of PC12 cells revealed that after treatment with PMA, PKCδ was translocated from the cytosol to the plasma membrane where PLD2 is mainly localized. These results suggest that PKCδ-dependent direct phosphorylation plays an important role in the regulation of PLD2 activity in PC12 cells.

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