Photo-induced adequate nitric oxide (PIANO)-mediated relaxation in isolated rabbit corpus cavernosum

Byungha Chung, Ki Churl Chang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

1. We further investigated our earlier proposal that NO- and NO2-carrying molecules potentiate photorelaxation using isolated rabbit corpus cavernosum. 2. Corporal smooth muscle, in the presence or absence of endothelium, relaxed only slightly upon u.v. light (366 nm) irradiation. But, NO- and/or NO2-containing compounds such as streptozotocin and NG-nitro-l-arginine methyl ester significantly (P < 0.01) enhanced photorelaxation in this tissue. In addition, NG-nitro-d-arginine methyl ester, known to lack inhibitory action on NO synthase, showed concentration-dependent potentiation of the photorelaxation. 3. Oxygen radical generating system via xanthine + xanthine oxidase and guanylate cyclase inhibitor, methylene blue, significantly (P < 0.05) inhibited the streptozotocin-potentiated photorelaxation. 4. Nitrite was accumulated by photolysis of streptozotocin, NG-nitro-l-arginine methyl ester and NG-nitro-d-arginine methyl ester, in a concentration and exposure time-dependent manner. 5. These observations indicate that NO is a potent relaxant of rabbit corpus cavernosum and further support our hypothesis that NO is released by photolysis from NO- and NO2-carrying molecules.

Original languageEnglish
Pages (from-to)893-898
Number of pages6
JournalGeneral Pharmacology
Volume25
Issue number5
DOIs
Publication statusPublished - 1994 Jan 1

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Nitric Oxide
Streptozocin
Rabbits
Photolysis
Xanthine
Xanthine Oxidase
Guanylate Cyclase
Methylene Blue
Nitrites
Nitric Oxide Synthase
Endothelium
Smooth Muscle
Reactive Oxygen Species
Light
arginine methyl ester

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

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title = "Photo-induced adequate nitric oxide (PIANO)-mediated relaxation in isolated rabbit corpus cavernosum",
abstract = "1. We further investigated our earlier proposal that NO- and NO2-carrying molecules potentiate photorelaxation using isolated rabbit corpus cavernosum. 2. Corporal smooth muscle, in the presence or absence of endothelium, relaxed only slightly upon u.v. light (366 nm) irradiation. But, NO- and/or NO2-containing compounds such as streptozotocin and NG-nitro-l-arginine methyl ester significantly (P < 0.01) enhanced photorelaxation in this tissue. In addition, NG-nitro-d-arginine methyl ester, known to lack inhibitory action on NO synthase, showed concentration-dependent potentiation of the photorelaxation. 3. Oxygen radical generating system via xanthine + xanthine oxidase and guanylate cyclase inhibitor, methylene blue, significantly (P < 0.05) inhibited the streptozotocin-potentiated photorelaxation. 4. Nitrite was accumulated by photolysis of streptozotocin, NG-nitro-l-arginine methyl ester and NG-nitro-d-arginine methyl ester, in a concentration and exposure time-dependent manner. 5. These observations indicate that NO is a potent relaxant of rabbit corpus cavernosum and further support our hypothesis that NO is released by photolysis from NO- and NO2-carrying molecules.",
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Photo-induced adequate nitric oxide (PIANO)-mediated relaxation in isolated rabbit corpus cavernosum. / Chung, Byungha; Chang, Ki Churl.

In: General Pharmacology, Vol. 25, No. 5, 01.01.1994, p. 893-898.

Research output: Contribution to journalArticle

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