Abstract
A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.
| Original language | English |
|---|---|
| Pages (from-to) | 1327-1333 |
| Number of pages | 7 |
| Journal | Journal of General Virology |
| Volume | 74 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1993 Jan 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Virology
Cite this
}
Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. / Fodor, E.; Seong, Baik Lin; Brownlee, G. G.
In: Journal of General Virology, Vol. 74, No. 7, 01.01.1993, p. 1327-1333.Research output: Contribution to journal › Article
TY - JOUR
T1 - Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter
AU - Fodor, E.
AU - Seong, Baik Lin
AU - Brownlee, G. G.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.
AB - A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.
UR - http://www.scopus.com/inward/record.url?scp=0027240154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027240154&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-74-7-1327
DO - 10.1099/0022-1317-74-7-1327
M3 - Article
VL - 74
SP - 1327
EP - 1333
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 7
ER -