Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter

E. Fodor, Baik Lin Seong, G. G. Brownlee

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.

Original languageEnglish
Pages (from-to)1327-1333
Number of pages7
JournalJournal of General Virology
Volume74
Issue number7
DOIs
Publication statusPublished - 1993 Jan 1

Fingerprint

Virion
Human Influenza
Nucleotides
Binding Sites
RNA
Point Mutation
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

@article{2458b2613f3347f2a828d0e8f8187faa,
title = "Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter",
abstract = "A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.",
author = "E. Fodor and Seong, {Baik Lin} and Brownlee, {G. G.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1099/0022-1317-74-7-1327",
language = "English",
volume = "74",
pages = "1327--1333",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "7",

}

TY - JOUR

T1 - Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter

AU - Fodor, E.

AU - Seong, Baik Lin

AU - Brownlee, G. G.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.

AB - A previous study of the 12 nucleotide-long influenza A virion RNA promoter has shown that three nucleotides, residues 9 to 11, were crucial for transcription in vitro, although other nucleotides play a significant but less important role. A model for polymerase-promoter recognition was proposed, according to which there were two sites: a binding site at residues 9 to 11 and a regulatory site at or near the site of initiation at residue 1. By studying the effect of point mutations in the promoter on the binding efficiency of the polymerase using a photochemical cross-linking assay, we now show that residues 9 to 12 are crucial for binding. In addition residues 4 to 8, though not as important, are involved in binding, possibly by stabilizing the polymerase-promoter complex. Both PB1 and PB2 apparently play an important role during virion RNA promoter recognition and binding.

UR - http://www.scopus.com/inward/record.url?scp=0027240154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027240154&partnerID=8YFLogxK

U2 - 10.1099/0022-1317-74-7-1327

DO - 10.1099/0022-1317-74-7-1327

M3 - Article

VL - 74

SP - 1327

EP - 1333

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 7

ER -