Photothermal spectral-domain optical coherence reflectometry for direct measurement of hemoglobin concentration of erythrocytes

Jinyeong Yim, Hun Kim, Suho Ryu, Sungwook Song, Hyun Ok Kim, Kyung A. Hyun, Hyo Il Jung, Chulmin Joo

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8 Citations (Scopus)


A novel optical detection method for hemoglobin concentration is described. The hemoglobin molecules consisting mainly of iron generate heat upon their absorption of light energy at 532. nm, which subsequently changes the refractive index of the blood. We exploit this photothermal effect to determine the hemoglobin concentration of erythrocytes without any preprocessing of blood. Highly sensitive measurement of refractive index alteration of blood samples is enabled by a spectral-domain low coherence reflectometric sensor with subnanometer-level optical path-length sensitivity. The performance and validity of the sensor are presented by comparing the measured results against the reference data acquired from an automatic hematology analyzer.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalBiosensors and Bioelectronics
Publication statusPublished - 2014 Jul 15


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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