A novel optical detection method for hemoglobin concentration is described. The hemoglobin molecules consisting mainly of iron generate heat upon their absorption of light energy at 532. nm, which subsequently changes the refractive index of the blood. We exploit this photothermal effect to determine the hemoglobin concentration of erythrocytes without any preprocessing of blood. Highly sensitive measurement of refractive index alteration of blood samples is enabled by a spectral-domain low coherence reflectometric sensor with subnanometer-level optical path-length sensitivity. The performance and validity of the sensor are presented by comparing the measured results against the reference data acquired from an automatic hematology analyzer.
Bibliographical noteFunding Information:
This research was supported in part by the research programs of the National Research Foundation of Korea (NRF) (MEST no. 2013-8-0622 , 2013-8-1806 and 2011–0016731 ) and the Center for BioNano Health-Guard funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Project (H-GUARD 2013M3A6B2078959 ).
All Science Journal Classification (ASJC) codes
- Biomedical Engineering