Physical characterization of cytochrome c- and vitamin B12-doped DNA thin films

Bramaramba Gnapareddy, Sreekantha Reddy Dugasani, Mallikarjuna Reddy Kesama, Kyunghwan Oh, Sung Ha Park

Research output: Contribution to journalArticle

Abstract

DNA can be useful in the field of biological and physical sciences due to its unique features, e.g., molecular recognition and self-assembly. Here, we fabricated cytochrome C (Cy)- and vitamin B12 (VB)-doped DNA thin films via a drop-casting method and explored their chemical and optoelectronic characteristics. Fourier transform infrared (FTIR), Raman, absorption, photoluminescence (PL), and electrical measurements were used to estimate critical concentrations of Cy and VB (where extremes of measured quantities occurred) in a fixed DNA concentration. These measurements focused on chemical interactions, binding modes, energy transfer mechanism, and electrical characteristics of the Cy- and VB-doped DNA thin films. We noticed that FTIR absorbance and Raman (UV–Vis) band intensities were increased (decreased), with an increase in the Cy concentration of up to 1.5 μM (= critical concentration of Cy) and a VB concentration of up to 3 mM (= critical concentration of VB) in DNA thin films. Significant band intensity changes and band position shifts of FTIR, Raman, and UV–Vis spectra were observed by varying concentrations of Cy and VB in DNA thin films. These changes provided the evidence of interaction between DNA and Cy (VB) via electrostatic bonding and intercalation interactions. The PL spectra exhibited strong red emissions at 612 nm. The changes of PL intensities revealed that effective energy transfer occurred between the VB and DNA up to critical concentration of VB, due to proper binding of VB. Finally, the current extremes for Cy- and VB-doped DNA thin films were observed at 1.5 μM of Cy and 3 mM of VB, respectively, which agreed well with the other characteristics.

Original languageEnglish
Article number121869
JournalMaterials Chemistry and Physics
Volume237
DOIs
Publication statusPublished - 2019 Nov 1

Fingerprint

vitamins
Vitamins
cytochromes
Vitamin B 12
Cytochromes c
DNA
deoxyribonucleic acid
Proteins
Thin films
thin films
Fourier transforms
Photoluminescence
Infrared radiation
photoluminescence
Energy transfer
electrostatic bonding
energy transfer
physical sciences
Molecular recognition
interactions

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Gnapareddy, Bramaramba ; Dugasani, Sreekantha Reddy ; Kesama, Mallikarjuna Reddy ; Oh, Kyunghwan ; Park, Sung Ha. / Physical characterization of cytochrome c- and vitamin B12-doped DNA thin films. In: Materials Chemistry and Physics. 2019 ; Vol. 237.
@article{4fc94a47ecc44947a457c4ac375150e1,
title = "Physical characterization of cytochrome c- and vitamin B12-doped DNA thin films",
abstract = "DNA can be useful in the field of biological and physical sciences due to its unique features, e.g., molecular recognition and self-assembly. Here, we fabricated cytochrome C (Cy)- and vitamin B12 (VB)-doped DNA thin films via a drop-casting method and explored their chemical and optoelectronic characteristics. Fourier transform infrared (FTIR), Raman, absorption, photoluminescence (PL), and electrical measurements were used to estimate critical concentrations of Cy and VB (where extremes of measured quantities occurred) in a fixed DNA concentration. These measurements focused on chemical interactions, binding modes, energy transfer mechanism, and electrical characteristics of the Cy- and VB-doped DNA thin films. We noticed that FTIR absorbance and Raman (UV–Vis) band intensities were increased (decreased), with an increase in the Cy concentration of up to 1.5 μM (= critical concentration of Cy) and a VB concentration of up to 3 mM (= critical concentration of VB) in DNA thin films. Significant band intensity changes and band position shifts of FTIR, Raman, and UV–Vis spectra were observed by varying concentrations of Cy and VB in DNA thin films. These changes provided the evidence of interaction between DNA and Cy (VB) via electrostatic bonding and intercalation interactions. The PL spectra exhibited strong red emissions at 612 nm. The changes of PL intensities revealed that effective energy transfer occurred between the VB and DNA up to critical concentration of VB, due to proper binding of VB. Finally, the current extremes for Cy- and VB-doped DNA thin films were observed at 1.5 μM of Cy and 3 mM of VB, respectively, which agreed well with the other characteristics.",
author = "Bramaramba Gnapareddy and Dugasani, {Sreekantha Reddy} and Kesama, {Mallikarjuna Reddy} and Kyunghwan Oh and Park, {Sung Ha}",
year = "2019",
month = "11",
day = "1",
doi = "10.1016/j.matchemphys.2019.121869",
language = "English",
volume = "237",
journal = "Materials Chemistry and Physics",
issn = "0254-0584",
publisher = "Elsevier BV",

}

Physical characterization of cytochrome c- and vitamin B12-doped DNA thin films. / Gnapareddy, Bramaramba; Dugasani, Sreekantha Reddy; Kesama, Mallikarjuna Reddy; Oh, Kyunghwan; Park, Sung Ha.

In: Materials Chemistry and Physics, Vol. 237, 121869, 01.11.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Physical characterization of cytochrome c- and vitamin B12-doped DNA thin films

AU - Gnapareddy, Bramaramba

AU - Dugasani, Sreekantha Reddy

AU - Kesama, Mallikarjuna Reddy

AU - Oh, Kyunghwan

AU - Park, Sung Ha

PY - 2019/11/1

Y1 - 2019/11/1

N2 - DNA can be useful in the field of biological and physical sciences due to its unique features, e.g., molecular recognition and self-assembly. Here, we fabricated cytochrome C (Cy)- and vitamin B12 (VB)-doped DNA thin films via a drop-casting method and explored their chemical and optoelectronic characteristics. Fourier transform infrared (FTIR), Raman, absorption, photoluminescence (PL), and electrical measurements were used to estimate critical concentrations of Cy and VB (where extremes of measured quantities occurred) in a fixed DNA concentration. These measurements focused on chemical interactions, binding modes, energy transfer mechanism, and electrical characteristics of the Cy- and VB-doped DNA thin films. We noticed that FTIR absorbance and Raman (UV–Vis) band intensities were increased (decreased), with an increase in the Cy concentration of up to 1.5 μM (= critical concentration of Cy) and a VB concentration of up to 3 mM (= critical concentration of VB) in DNA thin films. Significant band intensity changes and band position shifts of FTIR, Raman, and UV–Vis spectra were observed by varying concentrations of Cy and VB in DNA thin films. These changes provided the evidence of interaction between DNA and Cy (VB) via electrostatic bonding and intercalation interactions. The PL spectra exhibited strong red emissions at 612 nm. The changes of PL intensities revealed that effective energy transfer occurred between the VB and DNA up to critical concentration of VB, due to proper binding of VB. Finally, the current extremes for Cy- and VB-doped DNA thin films were observed at 1.5 μM of Cy and 3 mM of VB, respectively, which agreed well with the other characteristics.

AB - DNA can be useful in the field of biological and physical sciences due to its unique features, e.g., molecular recognition and self-assembly. Here, we fabricated cytochrome C (Cy)- and vitamin B12 (VB)-doped DNA thin films via a drop-casting method and explored their chemical and optoelectronic characteristics. Fourier transform infrared (FTIR), Raman, absorption, photoluminescence (PL), and electrical measurements were used to estimate critical concentrations of Cy and VB (where extremes of measured quantities occurred) in a fixed DNA concentration. These measurements focused on chemical interactions, binding modes, energy transfer mechanism, and electrical characteristics of the Cy- and VB-doped DNA thin films. We noticed that FTIR absorbance and Raman (UV–Vis) band intensities were increased (decreased), with an increase in the Cy concentration of up to 1.5 μM (= critical concentration of Cy) and a VB concentration of up to 3 mM (= critical concentration of VB) in DNA thin films. Significant band intensity changes and band position shifts of FTIR, Raman, and UV–Vis spectra were observed by varying concentrations of Cy and VB in DNA thin films. These changes provided the evidence of interaction between DNA and Cy (VB) via electrostatic bonding and intercalation interactions. The PL spectra exhibited strong red emissions at 612 nm. The changes of PL intensities revealed that effective energy transfer occurred between the VB and DNA up to critical concentration of VB, due to proper binding of VB. Finally, the current extremes for Cy- and VB-doped DNA thin films were observed at 1.5 μM of Cy and 3 mM of VB, respectively, which agreed well with the other characteristics.

UR - http://www.scopus.com/inward/record.url?scp=85068917658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068917658&partnerID=8YFLogxK

U2 - 10.1016/j.matchemphys.2019.121869

DO - 10.1016/j.matchemphys.2019.121869

M3 - Article

VL - 237

JO - Materials Chemistry and Physics

JF - Materials Chemistry and Physics

SN - 0254-0584

M1 - 121869

ER -