Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate

Tae Jin Kim; Su Jin Kim; Hyo Il Jung

doi:10.1007/s10404-008-0310-8

Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate

Tae Jin Kim, Su Jin Kim, Hyo Il Jung

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads. Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can be used as a physical stimulation factor and affect cell growth behaviors.

Original language	English
Pages (from-to)	131-138
Number of pages	8
Journal	Microfluidics and Nanofluidics
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/s10404-008-0310-8
Publication status	Published - 2009

Bibliographical note

Funding Information:
Acknowledgments This work was supported by ICBIN of Seoul R&BD program (Grant no. 10816) and National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science and Engineering Foundation (Grant no. R15-2004-024-01001-0).

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Materials Chemistry

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title = "Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate",

abstract = "Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads. Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can be used as a physical stimulation factor and affect cell growth behaviors.",

author = "Kim, {Tae Jin} and Kim, {Su Jin} and Jung, {Hyo Il}",

note = "Funding Information: Acknowledgments This work was supported by ICBIN of Seoul R&BD program (Grant no. 10816) and National Core Research Center (NCRC) for Nanomedical Technology of the Korea Science and Engineering Foundation (Grant no. R15-2004-024-01001-0).",

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