Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate

Tae Jin Kim, Su Jin Kim, Hyo il Jung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads. Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can be used as a physical stimulation factor and affect cell growth behaviors.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalMicrofluidics and Nanofluidics
Volume6
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

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stimulation
Microfluidics
beads
Cells
Polystyrenes
Cell growth
Polydimethylsiloxane
cells
Cell culture
Culture Media
Microscopes
cell culturing
culture media
microfluidic devices
cultured cells
Experiments
polystyrene
chambers
microscopes
intervals

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

Cite this

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Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate. / Kim, Tae Jin; Kim, Su Jin; Jung, Hyo il.

In: Microfluidics and Nanofluidics, Vol. 6, No. 1, 01.01.2009, p. 131-138.

Research output: Contribution to journalArticle

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