Abstract
Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads. Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can be used as a physical stimulation factor and affect cell growth behaviors.
| Original language | English |
|---|---|
| Pages (from-to) | 131-138 |
| Number of pages | 8 |
| Journal | Microfluidics and Nanofluidics |
| Volume | 6 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Materials Chemistry
Cite this
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Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate. / Kim, Tae Jin; Kim, Su Jin; Jung, Hyo Il.
In: Microfluidics and Nanofluidics, Vol. 6, No. 1, 01.01.2009, p. 131-138.Research output: Contribution to journal › Article
TY - JOUR
T1 - Physical stimulation of mammalian cells using micro-bead impact within a microfluidic environment to enhance growth rate
AU - Kim, Tae Jin
AU - Kim, Su Jin
AU - Jung, Hyo Il
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads. Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can be used as a physical stimulation factor and affect cell growth behaviors.
AB - Various cell stimulation experiments have been traditionally conducted regarding observation of cells to specific stimulative factors, and in light of this area of study, we report a new method by utilizing micro-beads. HeLa cells and MC3T3 cells are cultured in straight PDMS (polydimethylsiloxane) microfluidic devices and are stimulated by sterilized polystyrene micro-beads. Cell culture medium either with or without micro-beads are introduced in microfluidic cell culturing chambers at a specific time interval, and stimulated cells are observed using an inverted microscope. The results show that cells exposed under micro-bead stimulation perform at a higher growth rate than those under normal conditions. This paper demonstrates that micro-beads can be used as a physical stimulation factor and affect cell growth behaviors.
UR - http://www.scopus.com/inward/record.url?scp=58249089478&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58249089478&partnerID=8YFLogxK
U2 - 10.1007/s10404-008-0310-8
DO - 10.1007/s10404-008-0310-8
M3 - Article
AN - SCOPUS:58249089478
VL - 6
SP - 131
EP - 138
JO - Microfluidics and Nanofluidics
JF - Microfluidics and Nanofluidics
SN - 1613-4982
IS - 1
ER -