Physicochemical study of ascorbic acid 2-glucoside loaded hyaluronic acid dissolving microneedles irradiated by electron beam and gamma ray

Suyong Kim, Jeongwon Lee, F. Lahiji Shayan, Seohyun Kim, Inyoung Huh, Yonghao Ma, Huisuk Yang, Geonwoo Kang, Hyungil Jung

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


A dissolving microneedle (DMN) patch encapsulated with ascorbic acid 2-glucoside (AA2G) in a needle-shaped hyaluronic acid (HA) backbone was fabricated and sterilized by electron beam (e-beam, 5–40 kGy) and gamma ray (γ-ray, 5–30 kGy). DMN structures maintained their morphologies and fracture force regardless of e-beam and γ-ray irradiation doses. Both e-beam (40 kGy) and γ-ray (20 and 30 kGy) met the product sterility requirements for cosmetics and vaccines; however, γ-ray irradiation significantly degraded the encapsulated AA2G, while e-beam maintained AA2G activity. Thus, an e-beam dose of 40 kGy, which satisfied the sterility requirements without loss of AA2G, is suitable for terminal sterilization of DMNs. Moreover, we confirmed that the optimized irradiation (e-beam, 40 kGy) did not affect dissolution rate and drug release profile of DMNs. Further, we confirmed that HA, the backbone polymer of DMNs, could be utilized as a stabilizer that inhibits degradation of encapsulated AA2G by irradiation. This detailed analysis can be developed further to optimize various biological drugs in transdermal drug delivery systems.

Original languageEnglish
Pages (from-to)297-303
Number of pages7
JournalCarbohydrate Polymers
Publication statusPublished - 2018 Jan 15

Bibliographical note

Funding Information:
This work was supported by the R&D program of MSIP/COMPA ( 2016K000225 , Development of minimal pain multi-micro lancets for one-touch-smart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C0365 ); the work was also partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2017.

Publisher Copyright:
© 2017 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Materials Chemistry
  • Polymers and Plastics
  • Organic Chemistry


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