Physicochemical study of ascorbic acid 2-glucoside loaded hyaluronic acid dissolving microneedles irradiated by electron beam and gamma ray

Suyong Kim; Jeongwon Lee; F. Lahiji Shayan; Seohyun Kim; Inyoung Huh; Yonghao Ma; Huisuk Yang; Geonwoo Kang; Hyungil Jung

doi:10.1016/j.carbpol.2017.10.044

Physicochemical study of ascorbic acid 2-glucoside loaded hyaluronic acid dissolving microneedles irradiated by electron beam and gamma ray

Suyong Kim, Jeongwon Lee, F. Lahiji Shayan, Seohyun Kim, Inyoung Huh, Yonghao Ma, Huisuk Yang, Geonwoo Kang, Hyungil Jung

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

A dissolving microneedle (DMN) patch encapsulated with ascorbic acid 2-glucoside (AA2G) in a needle-shaped hyaluronic acid (HA) backbone was fabricated and sterilized by electron beam (e-beam, 5–40 kGy) and gamma ray (γ-ray, 5–30 kGy). DMN structures maintained their morphologies and fracture force regardless of e-beam and γ-ray irradiation doses. Both e-beam (40 kGy) and γ-ray (20 and 30 kGy) met the product sterility requirements for cosmetics and vaccines; however, γ-ray irradiation significantly degraded the encapsulated AA2G, while e-beam maintained AA2G activity. Thus, an e-beam dose of 40 kGy, which satisfied the sterility requirements without loss of AA2G, is suitable for terminal sterilization of DMNs. Moreover, we confirmed that the optimized irradiation (e-beam, 40 kGy) did not affect dissolution rate and drug release profile of DMNs. Further, we confirmed that HA, the backbone polymer of DMNs, could be utilized as a stabilizer that inhibits degradation of encapsulated AA2G by irradiation. This detailed analysis can be developed further to optimize various biological drugs in transdermal drug delivery systems.

Original language	English
Pages (from-to)	297-303
Number of pages	7
Journal	Carbohydrate Polymers
Volume	180
DOIs	https://doi.org/10.1016/j.carbpol.2017.10.044
Publication status	Published - 2018 Jan 15

Bibliographical note

Funding Information:
This work was supported by the R&D program of MSIP/COMPA ( 2016K000225 , Development of minimal pain multi-micro lancets for one-touch-smart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C0365 ); the work was also partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2017.

Publisher Copyright:
© 2017 Elsevier Ltd

All Science Journal Classification (ASJC) codes

Materials Chemistry
Polymers and Plastics
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.carbpol.2017.10.044

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title = "Physicochemical study of ascorbic acid 2-glucoside loaded hyaluronic acid dissolving microneedles irradiated by electron beam and gamma ray",

abstract = "A dissolving microneedle (DMN) patch encapsulated with ascorbic acid 2-glucoside (AA2G) in a needle-shaped hyaluronic acid (HA) backbone was fabricated and sterilized by electron beam (e-beam, 5–40 kGy) and gamma ray (γ-ray, 5–30 kGy). DMN structures maintained their morphologies and fracture force regardless of e-beam and γ-ray irradiation doses. Both e-beam (40 kGy) and γ-ray (20 and 30 kGy) met the product sterility requirements for cosmetics and vaccines; however, γ-ray irradiation significantly degraded the encapsulated AA2G, while e-beam maintained AA2G activity. Thus, an e-beam dose of 40 kGy, which satisfied the sterility requirements without loss of AA2G, is suitable for terminal sterilization of DMNs. Moreover, we confirmed that the optimized irradiation (e-beam, 40 kGy) did not affect dissolution rate and drug release profile of DMNs. Further, we confirmed that HA, the backbone polymer of DMNs, could be utilized as a stabilizer that inhibits degradation of encapsulated AA2G by irradiation. This detailed analysis can be developed further to optimize various biological drugs in transdermal drug delivery systems.",

author = "Suyong Kim and Jeongwon Lee and Shayan, {F. Lahiji} and Seohyun Kim and Inyoung Huh and Yonghao Ma and Huisuk Yang and Geonwoo Kang and Hyungil Jung",

note = "Funding Information: This work was supported by the R&D program of MSIP/COMPA ( 2016K000225 , Development of minimal pain multi-micro lancets for one-touch-smart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C0365 ); the work was also partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2017. Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

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doi = "10.1016/j.carbpol.2017.10.044",

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AU - Kim, Suyong

AU - Lee, Jeongwon

AU - Shayan, F. Lahiji

AU - Kim, Seohyun

AU - Huh, Inyoung

AU - Ma, Yonghao

AU - Yang, Huisuk

AU - Kang, Geonwoo

AU - Jung, Hyungil

N1 - Funding Information: This work was supported by the R&D program of MSIP/COMPA ( 2016K000225 , Development of minimal pain multi-micro lancets for one-touch-smart diagnostic sensor) and by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C0365 ); the work was also partially supported by the Graduate School of Yonsei University Research Scholarship Grants in 2017. Publisher Copyright: © 2017 Elsevier Ltd

PY - 2018/1/15

Y1 - 2018/1/15

N2 - A dissolving microneedle (DMN) patch encapsulated with ascorbic acid 2-glucoside (AA2G) in a needle-shaped hyaluronic acid (HA) backbone was fabricated and sterilized by electron beam (e-beam, 5–40 kGy) and gamma ray (γ-ray, 5–30 kGy). DMN structures maintained their morphologies and fracture force regardless of e-beam and γ-ray irradiation doses. Both e-beam (40 kGy) and γ-ray (20 and 30 kGy) met the product sterility requirements for cosmetics and vaccines; however, γ-ray irradiation significantly degraded the encapsulated AA2G, while e-beam maintained AA2G activity. Thus, an e-beam dose of 40 kGy, which satisfied the sterility requirements without loss of AA2G, is suitable for terminal sterilization of DMNs. Moreover, we confirmed that the optimized irradiation (e-beam, 40 kGy) did not affect dissolution rate and drug release profile of DMNs. Further, we confirmed that HA, the backbone polymer of DMNs, could be utilized as a stabilizer that inhibits degradation of encapsulated AA2G by irradiation. This detailed analysis can be developed further to optimize various biological drugs in transdermal drug delivery systems.

AB - A dissolving microneedle (DMN) patch encapsulated with ascorbic acid 2-glucoside (AA2G) in a needle-shaped hyaluronic acid (HA) backbone was fabricated and sterilized by electron beam (e-beam, 5–40 kGy) and gamma ray (γ-ray, 5–30 kGy). DMN structures maintained their morphologies and fracture force regardless of e-beam and γ-ray irradiation doses. Both e-beam (40 kGy) and γ-ray (20 and 30 kGy) met the product sterility requirements for cosmetics and vaccines; however, γ-ray irradiation significantly degraded the encapsulated AA2G, while e-beam maintained AA2G activity. Thus, an e-beam dose of 40 kGy, which satisfied the sterility requirements without loss of AA2G, is suitable for terminal sterilization of DMNs. Moreover, we confirmed that the optimized irradiation (e-beam, 40 kGy) did not affect dissolution rate and drug release profile of DMNs. Further, we confirmed that HA, the backbone polymer of DMNs, could be utilized as a stabilizer that inhibits degradation of encapsulated AA2G by irradiation. This detailed analysis can be developed further to optimize various biological drugs in transdermal drug delivery systems.

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ER -

Physicochemical study of ascorbic acid 2-glucoside loaded hyaluronic acid dissolving microneedles irradiated by electron beam and gamma ray

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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