Physiological function of NbRanBP1 in Nicotiana benthamiana

Hui Kyung Cho, Jong A. Park, Hyun Sook Pai

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.

Original languageEnglish
Pages (from-to)270-277
Number of pages8
JournalMolecules and Cells
Volume26
Issue number3
Publication statusPublished - 2008 Sep 30

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Gene Silencing
Cytosol
Tobacco
ran GTP-Binding Protein
Growth Disorders
Viruses
Mitochondrial Membrane Potential
Nuclear Envelope
Genes
Mammals
Reactive Oxygen Species
Carrier Proteins
Proteins
Yeasts

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Cho, Hui Kyung ; Park, Jong A. ; Pai, Hyun Sook. / Physiological function of NbRanBP1 in Nicotiana benthamiana. In: Molecules and Cells. 2008 ; Vol. 26, No. 3. pp. 270-277.
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Physiological function of NbRanBP1 in Nicotiana benthamiana. / Cho, Hui Kyung; Park, Jong A.; Pai, Hyun Sook.

In: Molecules and Cells, Vol. 26, No. 3, 30.09.2008, p. 270-277.

Research output: Contribution to journalArticle

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