Phytocalpain controls the proliferation and differentiation fates of cells in plant organ development

Joon Woo Ahn, Moonil Kim, Jeong Hwa Lim, Gyung Tae Kim, Hyunsook Pai

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Calpain, a calcium-dependent cysteine protease, plays an essential role in basic cellular processes in animal cells, including cell proliferation, apoptosis, and differentiation. NbDEK encodes the calpain homolog of N. benthamiana. In this study, virus-induced gene silencing (VIGS) of NbDEK resulted in arrested organ development and hyperplasia in all the major plant organs examined. The epidermal layers of the leaves and stems were covered with hyperproliferating cell masses, and stomata and trichome development was severely inhibited. During flower development, a single dome-like structure was grown from the flower meristem to generate a large cylinder-shaped flower lacking any floral organs. At the cellular level, cell division was sustained in tissues that were otherwise already differentiated, and cell differentiation was severely hampered. NbDEK is ubiquitously expressed in all the plant tissues examined. In the abnormal organs of the NbDEK VIGS lines, protein levels of D-type cyclins (CycD)2, CycD3, and proliferating cell nuclear antigen (PCNA) were greatly elevated, and transcription of E2F (E2 promoter binding factor), E2F-regulated genes, retinoblastoma (Rb), and KNOTTED1 (KN1)-type homeobox genes was also stimulated. These results suggest that phytocalpain is a key regulator of cell proliferation and differentiation during plant organogenesis, and that it acts partly by controlling the CycD/Rb pathway.

Original languageEnglish
Pages (from-to)969-981
Number of pages13
JournalPlant Journal
Volume38
Issue number6
DOIs
Publication statusPublished - 2004 Jun 1

Fingerprint

Plant Organogenesis
calpain
gene silencing
plant organs
cell differentiation
Cell Differentiation
cell proliferation
Calpain
Gene Silencing
flowers
viruses
proliferating cell nuclear antigen
homeotic genes
cysteine proteinases
cyclins
organogenesis
Cell Proliferation
hyperplasia
meristems
stomata

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Ahn, Joon Woo ; Kim, Moonil ; Lim, Jeong Hwa ; Kim, Gyung Tae ; Pai, Hyunsook. / Phytocalpain controls the proliferation and differentiation fates of cells in plant organ development. In: Plant Journal. 2004 ; Vol. 38, No. 6. pp. 969-981.
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Phytocalpain controls the proliferation and differentiation fates of cells in plant organ development. / Ahn, Joon Woo; Kim, Moonil; Lim, Jeong Hwa; Kim, Gyung Tae; Pai, Hyunsook.

In: Plant Journal, Vol. 38, No. 6, 01.06.2004, p. 969-981.

Research output: Contribution to journalArticle

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