Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors

Ji Hong Bong, Tae Hun Kim, Jaeyong Jung, Soo Jeong Lee, Jeong Soo Sung, Chang Kyu Lee, Min Jung Kang, Hyun Ok Kim, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Anti-coronavirusdisease-2019 (COVID-19; anti-severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARS-CoV-2 NPs was estimated to be 0.26 ± 0.05 % (absolute amount of 143.4 ± 25.2 ng, n=5) from 1 mL of pig sera. The binding activities of the isolated antibodies were confirmed using immunoassay and immunostaining. Based on the specific binding activity to NPs, a quantitative assay was performed using a surface plasmon resonance (SPR) biosensor. From the doseresponse curve, the binding constant (Kd) was calculated to be 185 pM and the limit of detection was estimated to be 1.02 pM. The SPR biosensor with the isolated antibodies against SARS-CoV-2 NPs was applied for the detection of SARS-CoV-2, MERS-CoV, and CoV strain 229E in culture fluid.

Original languageEnglish
Pages (from-to)358-368
Number of pages11
JournalBiochip Journal
Volume14
Issue number4
DOIs
Publication statusPublished - 2020 Dec

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187].

Publisher Copyright:
© 2020, The Korean BioChip Society and Springer.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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