Abstract
Scope: Piperonal is an aromatic compound found in vanilla and has a floral odor resembling vanillin. This study was aimed to test whether piperonal attenuates visceral adiposity induced by a high-fat diet (HFD) in mice and to explore the underlying molecular mechanisms. Methods and results: Male C57BL/6N mice were fed a normal diet, HFD, or 0.05% piperonal-supplemented HFD (PSD) for 10 weeks. PSD-fed mice showed attenuation of body weight gain, total visceral fat pad weights, and plasma lipid levels compared to HFD-fed mice. Piperonal supplementation of the HFD increased the mRNA expression of certain isotypes of adenylate cyclase (Adcy) and protein kinase A (PKA) in the white adipose tissue (WAT) of mice. The adipogenesis-related genes were downregulated, whereas fatty acid oxidation- and thermogenesis-related genes were upregulated in the WAT of PSD-fed mice compared to those in HFD-fed mice. Piperonal directly activated Adcy by decreasing the Km for its substrate (ATP) in plasma membranes prepared from the WAT of mice. Furthermore, piperonal-induced inhibition of adipocyte differentiation and elevation of Adcy and PKA activities in 3T3-L1 cells were abrogated by an Adcy inhibitor. Conclusion: The anti-adipogenic effect of piperonal in mice fed the high-fat diet appears to be associated with increased Adcy–PKA signaling in WAT.
| Original language | English |
|---|---|
| Article number | 1601124 |
| Journal | Molecular Nutrition and Food Research |
| Volume | 61 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2017 Nov 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Food Science
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Piperonal attenuates visceral adiposity in mice fed a high-fat diet : potential involvement of the adenylate cyclase–protein kinase A dependent pathway. / Chu, Songyi; Narayan, Vikram P.; Sung, Mi Kyung; Park, Taesun.
In: Molecular Nutrition and Food Research, Vol. 61, No. 11, 1601124, 01.11.2017.Research output: Contribution to journal › Article
TY - JOUR
T1 - Piperonal attenuates visceral adiposity in mice fed a high-fat diet
T2 - potential involvement of the adenylate cyclase–protein kinase A dependent pathway
AU - Chu, Songyi
AU - Narayan, Vikram P.
AU - Sung, Mi Kyung
AU - Park, Taesun
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Scope: Piperonal is an aromatic compound found in vanilla and has a floral odor resembling vanillin. This study was aimed to test whether piperonal attenuates visceral adiposity induced by a high-fat diet (HFD) in mice and to explore the underlying molecular mechanisms. Methods and results: Male C57BL/6N mice were fed a normal diet, HFD, or 0.05% piperonal-supplemented HFD (PSD) for 10 weeks. PSD-fed mice showed attenuation of body weight gain, total visceral fat pad weights, and plasma lipid levels compared to HFD-fed mice. Piperonal supplementation of the HFD increased the mRNA expression of certain isotypes of adenylate cyclase (Adcy) and protein kinase A (PKA) in the white adipose tissue (WAT) of mice. The adipogenesis-related genes were downregulated, whereas fatty acid oxidation- and thermogenesis-related genes were upregulated in the WAT of PSD-fed mice compared to those in HFD-fed mice. Piperonal directly activated Adcy by decreasing the Km for its substrate (ATP) in plasma membranes prepared from the WAT of mice. Furthermore, piperonal-induced inhibition of adipocyte differentiation and elevation of Adcy and PKA activities in 3T3-L1 cells were abrogated by an Adcy inhibitor. Conclusion: The anti-adipogenic effect of piperonal in mice fed the high-fat diet appears to be associated with increased Adcy–PKA signaling in WAT.
AB - Scope: Piperonal is an aromatic compound found in vanilla and has a floral odor resembling vanillin. This study was aimed to test whether piperonal attenuates visceral adiposity induced by a high-fat diet (HFD) in mice and to explore the underlying molecular mechanisms. Methods and results: Male C57BL/6N mice were fed a normal diet, HFD, or 0.05% piperonal-supplemented HFD (PSD) for 10 weeks. PSD-fed mice showed attenuation of body weight gain, total visceral fat pad weights, and plasma lipid levels compared to HFD-fed mice. Piperonal supplementation of the HFD increased the mRNA expression of certain isotypes of adenylate cyclase (Adcy) and protein kinase A (PKA) in the white adipose tissue (WAT) of mice. The adipogenesis-related genes were downregulated, whereas fatty acid oxidation- and thermogenesis-related genes were upregulated in the WAT of PSD-fed mice compared to those in HFD-fed mice. Piperonal directly activated Adcy by decreasing the Km for its substrate (ATP) in plasma membranes prepared from the WAT of mice. Furthermore, piperonal-induced inhibition of adipocyte differentiation and elevation of Adcy and PKA activities in 3T3-L1 cells were abrogated by an Adcy inhibitor. Conclusion: The anti-adipogenic effect of piperonal in mice fed the high-fat diet appears to be associated with increased Adcy–PKA signaling in WAT.
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UR - http://www.scopus.com/inward/citedby.url?scp=85032688693&partnerID=8YFLogxK
U2 - 10.1002/mnfr.201601124
DO - 10.1002/mnfr.201601124
M3 - Article
C2 - 28699236
AN - SCOPUS:85032688693
VL - 61
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
SN - 1613-4125
IS - 11
M1 - 1601124
ER -