Piperonal attenuates visceral adiposity in mice fed a high-fat diet: potential involvement of the adenylate cyclase–protein kinase A dependent pathway

Songyi Chu, Vikram P. Narayan, Mi Kyung Sung, Taesun Park

Research output: Contribution to journalArticle

Abstract

Scope: Piperonal is an aromatic compound found in vanilla and has a floral odor resembling vanillin. This study was aimed to test whether piperonal attenuates visceral adiposity induced by a high-fat diet (HFD) in mice and to explore the underlying molecular mechanisms. Methods and results: Male C57BL/6N mice were fed a normal diet, HFD, or 0.05% piperonal-supplemented HFD (PSD) for 10 weeks. PSD-fed mice showed attenuation of body weight gain, total visceral fat pad weights, and plasma lipid levels compared to HFD-fed mice. Piperonal supplementation of the HFD increased the mRNA expression of certain isotypes of adenylate cyclase (Adcy) and protein kinase A (PKA) in the white adipose tissue (WAT) of mice. The adipogenesis-related genes were downregulated, whereas fatty acid oxidation- and thermogenesis-related genes were upregulated in the WAT of PSD-fed mice compared to those in HFD-fed mice. Piperonal directly activated Adcy by decreasing the Km for its substrate (ATP) in plasma membranes prepared from the WAT of mice. Furthermore, piperonal-induced inhibition of adipocyte differentiation and elevation of Adcy and PKA activities in 3T3-L1 cells were abrogated by an Adcy inhibitor. Conclusion: The anti-adipogenic effect of piperonal in mice fed the high-fat diet appears to be associated with increased Adcy–PKA signaling in WAT.

Original languageEnglish
Article number1601124
JournalMolecular Nutrition and Food Research
Volume61
Issue number11
DOIs
Publication statusPublished - 2017 Nov 1

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adenylate kinase
Adenylate Kinase
Adiposity
adiposity
High Fat Diet
high fat diet
White Adipose Tissue
mice
white adipose tissue
adenylate cyclase
Adenylyl Cyclases
cAMP-dependent protein kinase
Cyclic AMP-Dependent Protein Kinases
Vanilla
3T3-L1 Cells
piperonal
Adipogenesis
visceral fat
beta oxidation
Intra-Abdominal Fat

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science

Cite this

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title = "Piperonal attenuates visceral adiposity in mice fed a high-fat diet: potential involvement of the adenylate cyclase–protein kinase A dependent pathway",
abstract = "Scope: Piperonal is an aromatic compound found in vanilla and has a floral odor resembling vanillin. This study was aimed to test whether piperonal attenuates visceral adiposity induced by a high-fat diet (HFD) in mice and to explore the underlying molecular mechanisms. Methods and results: Male C57BL/6N mice were fed a normal diet, HFD, or 0.05{\%} piperonal-supplemented HFD (PSD) for 10 weeks. PSD-fed mice showed attenuation of body weight gain, total visceral fat pad weights, and plasma lipid levels compared to HFD-fed mice. Piperonal supplementation of the HFD increased the mRNA expression of certain isotypes of adenylate cyclase (Adcy) and protein kinase A (PKA) in the white adipose tissue (WAT) of mice. The adipogenesis-related genes were downregulated, whereas fatty acid oxidation- and thermogenesis-related genes were upregulated in the WAT of PSD-fed mice compared to those in HFD-fed mice. Piperonal directly activated Adcy by decreasing the Km for its substrate (ATP) in plasma membranes prepared from the WAT of mice. Furthermore, piperonal-induced inhibition of adipocyte differentiation and elevation of Adcy and PKA activities in 3T3-L1 cells were abrogated by an Adcy inhibitor. Conclusion: The anti-adipogenic effect of piperonal in mice fed the high-fat diet appears to be associated with increased Adcy–PKA signaling in WAT.",
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Piperonal attenuates visceral adiposity in mice fed a high-fat diet : potential involvement of the adenylate cyclase–protein kinase A dependent pathway. / Chu, Songyi; Narayan, Vikram P.; Sung, Mi Kyung; Park, Taesun.

In: Molecular Nutrition and Food Research, Vol. 61, No. 11, 1601124, 01.11.2017.

Research output: Contribution to journalArticle

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T1 - Piperonal attenuates visceral adiposity in mice fed a high-fat diet

T2 - potential involvement of the adenylate cyclase–protein kinase A dependent pathway

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AU - Narayan, Vikram P.

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AB - Scope: Piperonal is an aromatic compound found in vanilla and has a floral odor resembling vanillin. This study was aimed to test whether piperonal attenuates visceral adiposity induced by a high-fat diet (HFD) in mice and to explore the underlying molecular mechanisms. Methods and results: Male C57BL/6N mice were fed a normal diet, HFD, or 0.05% piperonal-supplemented HFD (PSD) for 10 weeks. PSD-fed mice showed attenuation of body weight gain, total visceral fat pad weights, and plasma lipid levels compared to HFD-fed mice. Piperonal supplementation of the HFD increased the mRNA expression of certain isotypes of adenylate cyclase (Adcy) and protein kinase A (PKA) in the white adipose tissue (WAT) of mice. The adipogenesis-related genes were downregulated, whereas fatty acid oxidation- and thermogenesis-related genes were upregulated in the WAT of PSD-fed mice compared to those in HFD-fed mice. Piperonal directly activated Adcy by decreasing the Km for its substrate (ATP) in plasma membranes prepared from the WAT of mice. Furthermore, piperonal-induced inhibition of adipocyte differentiation and elevation of Adcy and PKA activities in 3T3-L1 cells were abrogated by an Adcy inhibitor. Conclusion: The anti-adipogenic effect of piperonal in mice fed the high-fat diet appears to be associated with increased Adcy–PKA signaling in WAT.

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