Piperonal prevents high-fat diet-induced hepatic steatosis and insulin resistance in mice via activation of adiponectin/AMPK pathway

X. Li, Y. Choi, Y. Yanakawa, T. Park

Research output: Contribution to journalArticle

9 Citations (Scopus)


Objective:Piperonal is an important flavor additive with antibacterial and anxiolytic properties. We investigated the effects and possible mechanisms of piperonal protection against high-fat diet (HFD)-induced hepatic steatosis and insulin resistance.Methods:C57BL/6 J mice were fed with a normal diet (ND; based on the AIN-76 rodent diet), HFD (20% fat and 1% cholesterol), or piperonal-supplemented diet (POD; HFD supplemented with 0.05% piperonal) for 10 weeks.Results:Piperonal supplementation reduced hepatic lipid concentrations, liver dysfunction and plasma levels of insulin and glucose in HFD-fed mice. Piperonal significantly enhanced mRNA expression and secretion of adiponectin in 3T3-L1 adipocytes. Dietary piperonal significantly increased circulating adiponectin levels and hepatic AMP-activated protein kinase (AMPK) activation in HFD-fed mice; these were associated with the suppression of sterol regulatory element binding protein-1c and activation of glucose transporter-2 translocation in the livers. Piperonal also significantly reduced the expression of endoplasmic reticulum (ER) stress markers in the livers of HFD-fed mice.Conclusions:Piperonal may activate the adiponectin/AMPK pathway in the livers of mice. The activated adiponectin/AMPK axis may inhibit p70S6 kinase signaling and the ER stress response, with protective effects on hepatic steatosis and insulin resistance.

Original languageEnglish
Pages (from-to)140-147
Number of pages8
JournalInternational Journal of Obesity
Issue number1
Publication statusPublished - 2014 Jan 1


All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

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