PKCθ expression in gastrointestinal stromal tumor

Kyoung Mee Kim, Dong Wook Kang, Woo Sung Moon, Jae Bok Park, Cheol Keun Park, Jin Hee Sohn, Jin Sook Jeong, Meeyon Cho, So Young Jin, Jong Sang Choi, Dae Young Kang

Research output: Contribution to journalArticle

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Abstract

Gastrointestinal stromal tumor is characterized by a gain of function mutation of KIT gene and the expression of c-kit protein, but in 5% of cases, c-kit expression is negative although histological findings of gastrointestinal stromal tumor are most suspicious. The existence of c-kit-negative gastrointestinal stromal tumors points to the need of additional markers for making the diagnosis. In this study, we studied the expression of PKCθ and correlated their expression with other immunohistochemical profiles of gastrointestinal stromal tumors and evaluated their usability as a diagnostic marker. For this purpose, 220 gastrointestinal stromal tumors were immunohistochemically stained for PKCθ, c-kit, CD34, α-smooth muscle actin and S-100 protein. Additionally, genetic studies of KIT and PDGFRA genes were performed using c-kit-negative or PKCθ-negative cases. All the 220 masses were either PKCθ-positive or c-kit-positive. PKCθ was positive in 212 (96%) cases and c-kit was positive in 216 (98%) cases in the cytoplasm of tumor cells with a diffuse staining pattern. Out of 212 PKCθ-positive GISTs, 208 (98%) cases were c-kit-positive, 174 (82%) cases were CD34-positive, 62 (29%) cases were SMA-positive and S-100 protein was positive in 54 cases (26%). Genetic analyses on eight PKCθ-negative cases showed exon 11 mutations of KIT gene in four cases. Two PKCθ-positive and c-kit-negative GISTs showed mutations of PDGFRA gene. Our study shows that PKCθ is a useful marker and it may play a role in the development of gastrointestinal stromal tumors. Together with c-kit, PKCθ immunostaining can be used as an important diagnostic tool in the pathologic diagnosis of gastrointestinal stromal tumors with its high specificity and sensitivity.

Original languageEnglish
Pages (from-to)1480-1486
Number of pages7
JournalModern Pathology
Volume19
Issue number11
DOIs
Publication statusPublished - 2006 Nov 4

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Gastrointestinal Stromal Tumors
S100 Proteins
Mutation
Proto-Oncogene Proteins c-kit
Genes
Smooth Muscle
Actins
Exons
Cytoplasm
Staining and Labeling
Gene Expression
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Kim, K. M., Kang, D. W., Moon, W. S., Park, J. B., Park, C. K., Sohn, J. H., ... Kang, D. Y. (2006). PKCθ expression in gastrointestinal stromal tumor. Modern Pathology, 19(11), 1480-1486. https://doi.org/10.1038/modpathol.3800673
Kim, Kyoung Mee ; Kang, Dong Wook ; Moon, Woo Sung ; Park, Jae Bok ; Park, Cheol Keun ; Sohn, Jin Hee ; Jeong, Jin Sook ; Cho, Meeyon ; Jin, So Young ; Choi, Jong Sang ; Kang, Dae Young. / PKCθ expression in gastrointestinal stromal tumor. In: Modern Pathology. 2006 ; Vol. 19, No. 11. pp. 1480-1486.
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abstract = "Gastrointestinal stromal tumor is characterized by a gain of function mutation of KIT gene and the expression of c-kit protein, but in 5{\%} of cases, c-kit expression is negative although histological findings of gastrointestinal stromal tumor are most suspicious. The existence of c-kit-negative gastrointestinal stromal tumors points to the need of additional markers for making the diagnosis. In this study, we studied the expression of PKCθ and correlated their expression with other immunohistochemical profiles of gastrointestinal stromal tumors and evaluated their usability as a diagnostic marker. For this purpose, 220 gastrointestinal stromal tumors were immunohistochemically stained for PKCθ, c-kit, CD34, α-smooth muscle actin and S-100 protein. Additionally, genetic studies of KIT and PDGFRA genes were performed using c-kit-negative or PKCθ-negative cases. All the 220 masses were either PKCθ-positive or c-kit-positive. PKCθ was positive in 212 (96{\%}) cases and c-kit was positive in 216 (98{\%}) cases in the cytoplasm of tumor cells with a diffuse staining pattern. Out of 212 PKCθ-positive GISTs, 208 (98{\%}) cases were c-kit-positive, 174 (82{\%}) cases were CD34-positive, 62 (29{\%}) cases were SMA-positive and S-100 protein was positive in 54 cases (26{\%}). Genetic analyses on eight PKCθ-negative cases showed exon 11 mutations of KIT gene in four cases. Two PKCθ-positive and c-kit-negative GISTs showed mutations of PDGFRA gene. Our study shows that PKCθ is a useful marker and it may play a role in the development of gastrointestinal stromal tumors. Together with c-kit, PKCθ immunostaining can be used as an important diagnostic tool in the pathologic diagnosis of gastrointestinal stromal tumors with its high specificity and sensitivity.",
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Kim, KM, Kang, DW, Moon, WS, Park, JB, Park, CK, Sohn, JH, Jeong, JS, Cho, M, Jin, SY, Choi, JS & Kang, DY 2006, 'PKCθ expression in gastrointestinal stromal tumor', Modern Pathology, vol. 19, no. 11, pp. 1480-1486. https://doi.org/10.1038/modpathol.3800673

PKCθ expression in gastrointestinal stromal tumor. / Kim, Kyoung Mee; Kang, Dong Wook; Moon, Woo Sung; Park, Jae Bok; Park, Cheol Keun; Sohn, Jin Hee; Jeong, Jin Sook; Cho, Meeyon; Jin, So Young; Choi, Jong Sang; Kang, Dae Young.

In: Modern Pathology, Vol. 19, No. 11, 04.11.2006, p. 1480-1486.

Research output: Contribution to journalArticle

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AU - Kim, Kyoung Mee

AU - Kang, Dong Wook

AU - Moon, Woo Sung

AU - Park, Jae Bok

AU - Park, Cheol Keun

AU - Sohn, Jin Hee

AU - Jeong, Jin Sook

AU - Cho, Meeyon

AU - Jin, So Young

AU - Choi, Jong Sang

AU - Kang, Dae Young

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N2 - Gastrointestinal stromal tumor is characterized by a gain of function mutation of KIT gene and the expression of c-kit protein, but in 5% of cases, c-kit expression is negative although histological findings of gastrointestinal stromal tumor are most suspicious. The existence of c-kit-negative gastrointestinal stromal tumors points to the need of additional markers for making the diagnosis. In this study, we studied the expression of PKCθ and correlated their expression with other immunohistochemical profiles of gastrointestinal stromal tumors and evaluated their usability as a diagnostic marker. For this purpose, 220 gastrointestinal stromal tumors were immunohistochemically stained for PKCθ, c-kit, CD34, α-smooth muscle actin and S-100 protein. Additionally, genetic studies of KIT and PDGFRA genes were performed using c-kit-negative or PKCθ-negative cases. All the 220 masses were either PKCθ-positive or c-kit-positive. PKCθ was positive in 212 (96%) cases and c-kit was positive in 216 (98%) cases in the cytoplasm of tumor cells with a diffuse staining pattern. Out of 212 PKCθ-positive GISTs, 208 (98%) cases were c-kit-positive, 174 (82%) cases were CD34-positive, 62 (29%) cases were SMA-positive and S-100 protein was positive in 54 cases (26%). Genetic analyses on eight PKCθ-negative cases showed exon 11 mutations of KIT gene in four cases. Two PKCθ-positive and c-kit-negative GISTs showed mutations of PDGFRA gene. Our study shows that PKCθ is a useful marker and it may play a role in the development of gastrointestinal stromal tumors. Together with c-kit, PKCθ immunostaining can be used as an important diagnostic tool in the pathologic diagnosis of gastrointestinal stromal tumors with its high specificity and sensitivity.

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Kim KM, Kang DW, Moon WS, Park JB, Park CK, Sohn JH et al. PKCθ expression in gastrointestinal stromal tumor. Modern Pathology. 2006 Nov 4;19(11):1480-1486. https://doi.org/10.1038/modpathol.3800673