Plakoglobin is a new target gene of histone deacetylase in human fibrosarcoma HT1080 cells

Joong Sup Shim, Dong Hoon Kim, Ho Jeong Kwon

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Histone deacetylase (HDAC) plays a key role in gene expression, by suppressing the transcription of a number of target genes. Identification of such genes is important for deciphering the functional role of HDAC. Here, using cancer gene-focused DNA microarray analysis, we identified plakoglobin as a new target gene of HDAC. Functional inhibition of HDAC by its specific inhibitors induced the expression of plakoglobin by eight-fold in human fibrosarcoma HT1080 cells. However, the expression of β-catenin, which is closely related to plakoglobin, was not altered, implying the specific function of HDAC in plakoglobin expression. Using antiacetyl-H4 antibody, chromatin immunoprecipitation analysis revealed that the distal region (-945 ∼ -646) of the promoter of plakoglobin is responsible for the HDAC-mediated repression of the gene. Moreover, the induced expression of plakoglobin by the inhibition of HDAC activated the Tcf/ Lef-dependent luciferase reporter gene, a well-known downstream effector of the Wnt signaling pathway. Furthermore, transient transfection of plakoglobin also activated Tcf/Lef reporter gene expression. Taken together, our results demonstrate that plakoglobin is a new target gene governed by HDAC, and that it acts as an oncogene in HT1080 cells.

Original languageEnglish
Pages (from-to)1704-1711
Number of pages8
JournalOncogene
Volume23
Issue number9
DOIs
Publication statusPublished - 2004 Mar 4

Fingerprint

gamma Catenin
Histone Deacetylases
Fibrosarcoma
Genes
Reporter Genes
Gene Expression
Catenins
Wnt Signaling Pathway
Chromatin Immunoprecipitation
Neoplasm Genes
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Luciferases
Oncogenes
Transfection

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Shim, Joong Sup ; Kim, Dong Hoon ; Kwon, Ho Jeong. / Plakoglobin is a new target gene of histone deacetylase in human fibrosarcoma HT1080 cells. In: Oncogene. 2004 ; Vol. 23, No. 9. pp. 1704-1711.
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Plakoglobin is a new target gene of histone deacetylase in human fibrosarcoma HT1080 cells. / Shim, Joong Sup; Kim, Dong Hoon; Kwon, Ho Jeong.

In: Oncogene, Vol. 23, No. 9, 04.03.2004, p. 1704-1711.

Research output: Contribution to journalArticle

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