Plant Flavonoid-Mediated Multifunctional Surface Modification Chemistry: Catechin Coating for Enhanced Osteogenesis of Human Stem Cells

Jung Seung Lee, Jong Seung Lee, Min Suk Lee, Soohwan An, Kisuk Yang, Kyueui Lee, Hee Seok Yang, Haeshin Lee, Seung Woo Cho

Research output: Contribution to journalArticlepeer-review

49 Citations (Scopus)


Application of surface chemistry using bioactive compounds enables simple functionalization of tissue-engineering scaffolds for improved biocompatibility and regenerative efficacy. Recently, surface modifications using natural polyphenols have been reported to serve as efficient multifunctional coating; however, there has yet to be any comprehensive application in tissue engineering. Here, we report a simple, multifunctional surface modification using catechin, a phenolic compound with many biological functions, found primarily in plants, to potentiate the functionality of polymeric scaffolds for bone regeneration by stem cells. We found that catechin hydrate can be efficiently deposited on the surface of various substrates and can greatly increase hydrophilicity of the substrates. While identifying the chemical mechanisms regulating catechin surface coating, we found that catechin molecules can self-assemble into dimers via cation-π interactions. Interestingly, the intrinsic biochemical functions of catechin coating provided the polymer scaffolds with antioxidative and calcium-binding abilities, resulting in enhanced adhesion, proliferation, mineralization, and osteogenic differentiation of human adipose-derived stem cells (hADSCs). Ultimately, catechin-functionalized polymer nanofiber scaffolds significantly promoted in vivo bone formation by hADSC transplantation in a critical-sized calvarial bone defect. Our study demonstrates that catechin can provide a biocompatible, multifunctional, and cost-effective surface modification chemistry to produce functional scaffolds with improved tissue regenerative efficacy.

Original languageEnglish
Pages (from-to)4375-4384
Number of pages10
JournalChemistry of Materials
Issue number10
Publication statusPublished - 2017 May 23

Bibliographical note

Funding Information:
This work was supported by a grant (2016R1A5A1004694) from the Translational Research Center for Protein Function Control funded by the Ministry of Science, ICT and Future Planning, Republic of Korea and a grant (2015R1A2A1A15053771) from the National Research Foundation (NRF), Republic of Korea. This work was supported by the Institute for Basic Science (IBS-R026-D1).

Publisher Copyright:
© 2017 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry


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