Polychlorinated biphenyls are toxic pollutants and their degradation is quite slow in the environment. Recently, interest in bioremediation using PCB-degrading bacteria has increased. In a previous report, plant terpenes (p-cymene, (S)-(-)-limonene, α-pynene, and α-terpinene) have been found to be utilized by a PCB degrader and to induce the biphenyl dioxygenase gene in pure culture. In this study, Pseudomonas pseudoalcaligenes KF707, a PCB-degrading Gram-negative soil bacterium, was used to determine whether the terpene stimulation of PCB degrader occurred in the natural environment. First, P. pseudoalcaligenes KF707 was genetically tagged using a transposon with gfp (green fluorescent protein) as a reporter gene. The population dynamics of P. pseudoalcaligenes KF707 harboring gfp gene in a PCB-contaminated environment was examined with or without terpenoids added to the microcosm. About 10-100-fold increase was found in the population of PCB degraders when terpene was added, compared with control (non-terpenes samples and biphenyl added samples). It was proposed that the gfp-monitoring system is very useful and terpenes enhance the survivability of PCB degraders in PCB-contaminated environments.
|Number of pages||6|
|Journal||Journal of microbiology and biotechnology|
|Publication status||Published - 2003 Jun|
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology