Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating

Hyok Jin Seo, Mi Hee Lee, Byeong Ju Kwon, Hye Lee Kim, Seung Jin Lee, Bong Jin Kim, Kang Kyun Wang, Yong Rok Kim, Jongchul Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Advanced biomaterials should also be bioactive with regard to desirable cellular responses, such as selective protein adsorption and cell attachment, proliferation, and differentiation. To enhance cell-material interactions, surface modifications have commonly been performed. Among the various surface modification approaches, atmospheric pressure glow discharge plasma has been used to change a hydrophobic polymer surface to a hydrophilic surface. Poly(L-lactic acid) (PLLA)-derived scaffolds lack cell recognition signals and the hydrophobic nature of PLLA hinders cell seeding. To make PLLA surfaces more conducive to cell attachment and spreading, surface modifications may be used to create cell-biomaterial interfaces that elicit controlled cell adhesion and maintain differentiated phenotypes. In this study, (He) gaseous atmospheric plasma glow discharge was used to change the characteristics of a 3D-type polymeric scaffold from hydrophobic to hydrophilic on both the outer and inner surfaces of the scaffold and the penetration efficiency with fibronectin was investigated. Field-emission scanning electron microscope images showed that some grooves were formed on the PLLA fibers after plasma treatment. X-ray photoelectron spectroscopy data also showed chemical changes in the PLLA structure. After plasma treatment, -CN (285.76 eV) was increased in C1s and -NH2 (399.70 eV) was increased significantly and -N=CH (400.80 eV) and -NH3 + (402.05 eV) were newly appeared in N1s. These changes allowed fibronectin to penetrate into the PLLA scaffold; this could be observed by confocal microscopy. In conclusion, helium atmospheric pressure plasma treatment was effective in modifying the polymeric scaffold, making it hydrophilic, and this treatment can also be used in tissue engineering research as needed to make polymers hydrophilic.

Original languageEnglish
Article number073304
JournalJournal of Applied Physics
Volume114
Issue number7
DOIs
Publication statusPublished - 2013 Aug 21

Fingerprint

lactic acid
proteins
coatings
cells
glow discharges
attachment
atmospheric pressure
phenotype
tissue engineering
polymers
inoculation
activity (biology)
grooves
surface reactions
field emission
adhesion
penetration
electron microscopes
helium
photoelectron spectroscopy

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

Cite this

Jin Seo, Hyok ; Hee Lee, Mi ; Kwon, Byeong Ju ; Kim, Hye Lee ; Jin Lee, Seung ; Kim, Bong Jin ; Wang, Kang Kyun ; Kim, Yong Rok ; Park, Jongchul. / Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating. In: Journal of Applied Physics. 2013 ; Vol. 114, No. 7.
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abstract = "Advanced biomaterials should also be bioactive with regard to desirable cellular responses, such as selective protein adsorption and cell attachment, proliferation, and differentiation. To enhance cell-material interactions, surface modifications have commonly been performed. Among the various surface modification approaches, atmospheric pressure glow discharge plasma has been used to change a hydrophobic polymer surface to a hydrophilic surface. Poly(L-lactic acid) (PLLA)-derived scaffolds lack cell recognition signals and the hydrophobic nature of PLLA hinders cell seeding. To make PLLA surfaces more conducive to cell attachment and spreading, surface modifications may be used to create cell-biomaterial interfaces that elicit controlled cell adhesion and maintain differentiated phenotypes. In this study, (He) gaseous atmospheric plasma glow discharge was used to change the characteristics of a 3D-type polymeric scaffold from hydrophobic to hydrophilic on both the outer and inner surfaces of the scaffold and the penetration efficiency with fibronectin was investigated. Field-emission scanning electron microscope images showed that some grooves were formed on the PLLA fibers after plasma treatment. X-ray photoelectron spectroscopy data also showed chemical changes in the PLLA structure. After plasma treatment, -CN (285.76 eV) was increased in C1s and -NH2 (399.70 eV) was increased significantly and -N=CH (400.80 eV) and -NH3 + (402.05 eV) were newly appeared in N1s. These changes allowed fibronectin to penetrate into the PLLA scaffold; this could be observed by confocal microscopy. In conclusion, helium atmospheric pressure plasma treatment was effective in modifying the polymeric scaffold, making it hydrophilic, and this treatment can also be used in tissue engineering research as needed to make polymers hydrophilic.",
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Plasma treatment induces internal surface modifications of electrospun poly(L-lactic) acid scaffold to enhance protein coating. / Jin Seo, Hyok; Hee Lee, Mi; Kwon, Byeong Ju; Kim, Hye Lee; Jin Lee, Seung; Kim, Bong Jin; Wang, Kang Kyun; Kim, Yong Rok; Park, Jongchul.

In: Journal of Applied Physics, Vol. 114, No. 7, 073304, 21.08.2013.

Research output: Contribution to journalArticle

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AU - Jin Seo, Hyok

AU - Hee Lee, Mi

AU - Kwon, Byeong Ju

AU - Kim, Hye Lee

AU - Jin Lee, Seung

AU - Kim, Bong Jin

AU - Wang, Kang Kyun

AU - Kim, Yong Rok

AU - Park, Jongchul

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