In this report, we investigate plasmon-enhanced imaging fluorescence correlation spectroscopy (p-FCS). p-FCS takes advantage of extreme light confinement by localization at nanogap-based plasmonic nanodimer arrays (PNAs) for enhanced signal-to-noise ratio (SNR) and improved precision by registration with surface plasmon microscopy images. Theoretical results corroborate the enhancement by PNAs in the far-field. Near-field scanning optical microscopy was used to confirm near-field localization experimentally. Experimental confirmation was also conducted with fluorescent nanobeads. The concept was further applied to studying the diffusion dynamics of lysosomes in HEK293T cells stimulated by phorbol 12-myristate 13-acetate treatment. It was found that lysosomes demonstrate stronger super-diffusive behavior with relatively weaker sub-diffusion after stimulation. SNR measured of p-FCS was improved by 9.77 times over conventional FCS. This report is expected to serve as the foundation for an enhanced analytical tool to explore subcellular dynamics.
Bibliographical noteFunding Information:
The authors are grateful for the support by the National Research Foundation (NRF) grants funded by the Korean Government ( 2019R1A4A1025958 ) and from the Korea Medical Device Development Fund (Project Number: 202011D25 ). W. J, Rhee and J.-S. Shin acknowledge the support by the NRF ( 2019R1A6A1A03032869 ).
© 2021 Elsevier B.V.
All Science Journal Classification (ASJC) codes
- Biomedical Engineering