Plasmon-enhanced fluorescence correlation spectroscopy for super-localized detection of nanoscale subcellular dynamics

Hongki Lee, Woo Joong Rhee, Gwiyeong Moon, Seongmin Im, Taehwang Son, Jeon Soo Shin, Donghyun Kim

Research output: Contribution to journalArticlepeer-review

Abstract

In this report, we investigate plasmon-enhanced imaging fluorescence correlation spectroscopy (p-FCS). p-FCS takes advantage of extreme light confinement by localization at nanogap-based plasmonic nanodimer arrays (PNAs) for enhanced signal-to-noise ratio (SNR) and improved precision by registration with surface plasmon microscopy images. Theoretical results corroborate the enhancement by PNAs in the far-field. Near-field scanning optical microscopy was used to confirm near-field localization experimentally. Experimental confirmation was also conducted with fluorescent nanobeads. The concept was further applied to studying the diffusion dynamics of lysosomes in HEK293T cells stimulated by phorbol 12-myristate 13-acetate treatment. It was found that lysosomes demonstrate stronger super-diffusive behavior with relatively weaker sub-diffusion after stimulation. SNR measured of p-FCS was improved by 9.77 times over conventional FCS. This report is expected to serve as the foundation for an enhanced analytical tool to explore subcellular dynamics.

Original languageEnglish
Article number113219
JournalBiosensors and Bioelectronics
Volume184
DOIs
Publication statusPublished - 2021 Jul 15

Bibliographical note

Funding Information:
The authors are grateful for the support by the National Research Foundation (NRF) grants funded by the Korean Government ( 2019R1A4A1025958 ) and from the Korea Medical Device Development Fund (Project Number: 202011D25 ). W. J, Rhee and J.-S. Shin acknowledge the support by the NRF ( 2019R1A6A1A03032869 ).

Publisher Copyright:
© 2021 Elsevier B.V.

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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