Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules

Wonju Lee, Youngjin Oh, Kyujung Kim, Yoshiaki Kinosita, Nagisa Mikami, Takayuki Nishizaka, Donghyun Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this presentation, we explore the feasibility of plasmonic nanohole-based sub-diffraction-limited nanoscopy for biomolecular imaging. The technique utilizes near-field distribution localized by surface plasmon localization on metallic nanoholes which is used to sample molecular fluorescence. The optimum geometry of nanohole arrays was determined by numerical analysis. The localization sampling was applied to reconstructing sub-diffraction-limited images of gliding microtubules with a 76 nm effective resolution in the lateral direction. Extraordinary light transmission was also employed to address enhancement of axial resolution using nanohole arrays, based on which extraction of gliding motions of bacteria was demonstrated with an axial resolution down to 50 nm.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
PublisherSPIE
Volume9721
ISBN (Electronic)9781628419559
DOIs
Publication statusPublished - 2016 Jan 1
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII - San Francisco, United States
Duration: 2016 Feb 152016 Feb 17

Other

OtherNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII
CountryUnited States
CitySan Francisco
Period16/2/1516/2/17

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Lee, W., Oh, Y., Kim, K., Kinosita, Y., Mikami, N., Nishizaka, T., & Kim, D. (2016). Plasmonic nanohole-based sub-diffraction-limited fluorescence microscopy for imaging of gliding biomolecules. In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII (Vol. 9721). [972107] SPIE. https://doi.org/10.1117/12.2212903