Plasmonics-based spatially activated light microscopy for super-resolution imaging of molecular fluorescence

Kyujung Kim, Youngjin Oh, Wonju Lee, Donghyun Kim

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In this Letter, we explore plasmonics-based spatially activated light microscopy [PSALM] for sub-diffraction-limited imaging of biomolecules. PSALM is based on the spatially switched activation of local amplified electromagnetic hot spots under multiple light incidence conditions. The hot spots are associated with surface plasmons that are excited and localized by surface nanostructures. The feasibility of the concept was demonstrated by imaging fluorescent nanobeads on a two-dimensional gold nanograting of a 100-nm-wide grating ridge, the size of which is the measure of the imaging resolution. The result confirms the performance of PSALM for imaging nanobeads at a resolution below the conventional diffraction limit.

Original languageEnglish
Pages (from-to)3501-3503
Number of pages3
JournalOptics Letters
Volume35
Issue number20
DOIs
Publication statusPublished - 2010 Oct 15

Fingerprint

microscopy
fluorescence
plasmons
diffraction
ridges
incidence
gratings
activation
gold
electromagnetism

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

@article{ba37fad95220433486a0d9c097f1b22f,
title = "Plasmonics-based spatially activated light microscopy for super-resolution imaging of molecular fluorescence",
abstract = "In this Letter, we explore plasmonics-based spatially activated light microscopy [PSALM] for sub-diffraction-limited imaging of biomolecules. PSALM is based on the spatially switched activation of local amplified electromagnetic hot spots under multiple light incidence conditions. The hot spots are associated with surface plasmons that are excited and localized by surface nanostructures. The feasibility of the concept was demonstrated by imaging fluorescent nanobeads on a two-dimensional gold nanograting of a 100-nm-wide grating ridge, the size of which is the measure of the imaging resolution. The result confirms the performance of PSALM for imaging nanobeads at a resolution below the conventional diffraction limit.",
author = "Kyujung Kim and Youngjin Oh and Wonju Lee and Donghyun Kim",
year = "2010",
month = "10",
day = "15",
doi = "10.1364/OL.35.003501",
language = "English",
volume = "35",
pages = "3501--3503",
journal = "Optics Letters",
issn = "0146-9592",
publisher = "The Optical Society",
number = "20",

}

Plasmonics-based spatially activated light microscopy for super-resolution imaging of molecular fluorescence. / Kim, Kyujung; Oh, Youngjin; Lee, Wonju; Kim, Donghyun.

In: Optics Letters, Vol. 35, No. 20, 15.10.2010, p. 3501-3503.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Plasmonics-based spatially activated light microscopy for super-resolution imaging of molecular fluorescence

AU - Kim, Kyujung

AU - Oh, Youngjin

AU - Lee, Wonju

AU - Kim, Donghyun

PY - 2010/10/15

Y1 - 2010/10/15

N2 - In this Letter, we explore plasmonics-based spatially activated light microscopy [PSALM] for sub-diffraction-limited imaging of biomolecules. PSALM is based on the spatially switched activation of local amplified electromagnetic hot spots under multiple light incidence conditions. The hot spots are associated with surface plasmons that are excited and localized by surface nanostructures. The feasibility of the concept was demonstrated by imaging fluorescent nanobeads on a two-dimensional gold nanograting of a 100-nm-wide grating ridge, the size of which is the measure of the imaging resolution. The result confirms the performance of PSALM for imaging nanobeads at a resolution below the conventional diffraction limit.

AB - In this Letter, we explore plasmonics-based spatially activated light microscopy [PSALM] for sub-diffraction-limited imaging of biomolecules. PSALM is based on the spatially switched activation of local amplified electromagnetic hot spots under multiple light incidence conditions. The hot spots are associated with surface plasmons that are excited and localized by surface nanostructures. The feasibility of the concept was demonstrated by imaging fluorescent nanobeads on a two-dimensional gold nanograting of a 100-nm-wide grating ridge, the size of which is the measure of the imaging resolution. The result confirms the performance of PSALM for imaging nanobeads at a resolution below the conventional diffraction limit.

UR - http://www.scopus.com/inward/record.url?scp=78549231943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78549231943&partnerID=8YFLogxK

U2 - 10.1364/OL.35.003501

DO - 10.1364/OL.35.003501

M3 - Article

VL - 35

SP - 3501

EP - 3503

JO - Optics Letters

JF - Optics Letters

SN - 0146-9592

IS - 20

ER -