Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells

Jin Eun Kim, Won Yoon Chung, Kyung Soo Chun, Chang Ki Lee, Hee Juhn Park, Won Bae Kim, Kwang Kyun Park

Research output: Contribution to journalArticle

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Abstract

To evaluate the anticarcinogenic activity of methanol extract of Pleurospermum kamtschaticum (PKE), we assessed its apoptosis-inducing capability in HT-29 colon carcinoma cells. PKE treatment for 2 h reduced cell viability in a dose-related manner, and induced apoptotic morphological changes. Flow cytometric analysis indicated that PKE treatment at 0.05 mg/ml induced early apoptosis in 66.2% of HT-29 cells. Additionally, Bcl-2 expression was substantially reduced in PKE-treated HT-29 cells, increasing the Bax/Bcl-2 ratio. The protein levels of procaspase-9 and procaspase-3 were decreased markedly, reflecting caspase-9 and caspase-3 activation, and resulting PARP cleavage was noted in the PKE-treated HT-29 cells. Furthermore, we detected increased NAG-1 expression in the PKEtreated HT-29 cells. In an in vivo study, intraperitoneal PKE administration suppressed the formation of tumor nodules in the lungs of mice. These results indicate that PKE can serve as a beneficial supplement in the treatment and the prevention of colon cancer.

Original languageEnglish
Pages (from-to)788-792
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number4
DOIs
Publication statusPublished - 2010 May 3

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HT29 Cells
Caspase 9
Caspase 3
Colonic Neoplasms
Cells
Apoptosis
Methanol
Tumors
Chemical activation
Cell Survival
Colon
Proteins
Carcinoma
Lung
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Kim, Jin Eun ; Chung, Won Yoon ; Chun, Kyung Soo ; Lee, Chang Ki ; Park, Hee Juhn ; Kim, Won Bae ; Park, Kwang Kyun. / Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells. In: Bioscience, Biotechnology and Biochemistry. 2010 ; Vol. 74, No. 4. pp. 788-792.
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Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells. / Kim, Jin Eun; Chung, Won Yoon; Chun, Kyung Soo; Lee, Chang Ki; Park, Hee Juhn; Kim, Won Bae; Park, Kwang Kyun.

In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 4, 03.05.2010, p. 788-792.

Research output: Contribution to journalArticle

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