pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea

Soon Kew Park, Jung Yoo Lee, Chulhun Ludgerus Chang, Min Ki Lee, Han Chul Son, Cheol Min Kim, Hyun Jung Jang, Hee Kyung Park, Seok Hoon Jeong

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30 Citations (Scopus)

Abstract

Background: Pyrazinamide (PZA) is among the first-line drugs for the treatment of tuberculosis. In vitro, it kills semidormant mycobacteria only at low pH. The purpose of this study was to compare PZA resistance with pyrazinamidase (PZase) activity and the genotype to better understand the molecular basis of PZA resistance and to expand the profile of pncA mutations worldwide. Results: Of the 28 tested strains of Mycobacterium tuberculosis, 6 were susceptible to PZA and positive for PZase activity and had no pncA mutations. Twenty-one strains were resistant to PZA and negative for PZase activity and had mutations in the pncA gene, including 15 point mutations, 5 insertions, and 2 deletions. One strain had no mutation in the pncA gene, even though it was resistant to PZA and negative for PZase activity. Three isolates had adenine to guanine point mutations in the -11 upstream region, making this the most common type of pncA mutations in this study, with at least two different RFLP patterns. Conclusion: These data help in the understanding of the molecular basis of PZA resistance. An adenine to guanine point mutation in the -11 upstream region was the most common type of pncA mutation in our isolates. The results of pncA mutation analyses should be carefully interpreted for epidemiologic purposes.

Original languageEnglish
Article number4
JournalBMC Infectious Diseases
Volume1
DOIs
Publication statusPublished - 2001 Jun 20

All Science Journal Classification (ASJC) codes

  • Infectious Diseases

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    Park, S. K., Lee, J. Y., Chang, C. L., Lee, M. K., Son, H. C., Kim, C. M., Jang, H. J., Park, H. K., & Jeong, S. H. (2001). pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea. BMC Infectious Diseases, 1, [4]. https://doi.org/10.1186/1471-2334-1-4