pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea

Soon Kew Park, Jung Yoo Lee, Chulhun Ludgerus Chang, Min Ki Lee, Han Chul Son, Cheol Min Kim, Hyun Jung Jang, Hee Kyung Park, Seokhoon Jeong

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Pyrazinamide (PZA) is among the first-line drugs for the treatment of tuberculosis. In vitro, it kills semidormant mycobacteria only at low pH. The purpose of this study was to compare PZA resistance with pyrazinamidase (PZase) activity and the genotype to better understand the molecular basis of PZA resistance and to expand the profile of pncA mutations worldwide. Results: Of the 28 tested strains of Mycobacterium tuberculosis, 6 were susceptible to PZA and positive for PZase activity and had no pncA mutations. Twenty-one strains were resistant to PZA and negative for PZase activity and had mutations in the pncA gene, including 15 point mutations, 5 insertions, and 2 deletions. One strain had no mutation in the pncA gene, even though it was resistant to PZA and negative for PZase activity. Three isolates had adenine to guanine point mutations in the -11 upstream region, making this the most common type of pncA mutations in this study, with at least two different RFLP patterns. Conclusion: These data help in the understanding of the molecular basis of PZA resistance. An adenine to guanine point mutation in the -11 upstream region was the most common type of pncA mutation in our isolates. The results of pncA mutation analyses should be carefully interpreted for epidemiologic purposes.

Original languageEnglish
Article number4
JournalBMC Infectious Diseases
Volume1
DOIs
Publication statusPublished - 2001 Jun 20

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Pyrazinamide
Korea
Mycobacterium tuberculosis
Mutation
Point Mutation
Guanine
Adenine
Mycobacterium
Restriction Fragment Length Polymorphisms
Genes
Tuberculosis
Genotype
pyrazinamide deamidase
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Infectious Diseases

Cite this

Park, Soon Kew ; Lee, Jung Yoo ; Chang, Chulhun Ludgerus ; Lee, Min Ki ; Son, Han Chul ; Kim, Cheol Min ; Jang, Hyun Jung ; Park, Hee Kyung ; Jeong, Seokhoon. / pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea. In: BMC Infectious Diseases. 2001 ; Vol. 1.
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abstract = "Background: Pyrazinamide (PZA) is among the first-line drugs for the treatment of tuberculosis. In vitro, it kills semidormant mycobacteria only at low pH. The purpose of this study was to compare PZA resistance with pyrazinamidase (PZase) activity and the genotype to better understand the molecular basis of PZA resistance and to expand the profile of pncA mutations worldwide. Results: Of the 28 tested strains of Mycobacterium tuberculosis, 6 were susceptible to PZA and positive for PZase activity and had no pncA mutations. Twenty-one strains were resistant to PZA and negative for PZase activity and had mutations in the pncA gene, including 15 point mutations, 5 insertions, and 2 deletions. One strain had no mutation in the pncA gene, even though it was resistant to PZA and negative for PZase activity. Three isolates had adenine to guanine point mutations in the -11 upstream region, making this the most common type of pncA mutations in this study, with at least two different RFLP patterns. Conclusion: These data help in the understanding of the molecular basis of PZA resistance. An adenine to guanine point mutation in the -11 upstream region was the most common type of pncA mutation in our isolates. The results of pncA mutation analyses should be carefully interpreted for epidemiologic purposes.",
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Park, SK, Lee, JY, Chang, CL, Lee, MK, Son, HC, Kim, CM, Jang, HJ, Park, HK & Jeong, S 2001, 'pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea', BMC Infectious Diseases, vol. 1, 4. https://doi.org/10.1186/1471-2334-1-4

pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea. / Park, Soon Kew; Lee, Jung Yoo; Chang, Chulhun Ludgerus; Lee, Min Ki; Son, Han Chul; Kim, Cheol Min; Jang, Hyun Jung; Park, Hee Kyung; Jeong, Seokhoon.

In: BMC Infectious Diseases, Vol. 1, 4, 20.06.2001.

Research output: Contribution to journalArticle

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T1 - pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea

AU - Park, Soon Kew

AU - Lee, Jung Yoo

AU - Chang, Chulhun Ludgerus

AU - Lee, Min Ki

AU - Son, Han Chul

AU - Kim, Cheol Min

AU - Jang, Hyun Jung

AU - Park, Hee Kyung

AU - Jeong, Seokhoon

PY - 2001/6/20

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N2 - Background: Pyrazinamide (PZA) is among the first-line drugs for the treatment of tuberculosis. In vitro, it kills semidormant mycobacteria only at low pH. The purpose of this study was to compare PZA resistance with pyrazinamidase (PZase) activity and the genotype to better understand the molecular basis of PZA resistance and to expand the profile of pncA mutations worldwide. Results: Of the 28 tested strains of Mycobacterium tuberculosis, 6 were susceptible to PZA and positive for PZase activity and had no pncA mutations. Twenty-one strains were resistant to PZA and negative for PZase activity and had mutations in the pncA gene, including 15 point mutations, 5 insertions, and 2 deletions. One strain had no mutation in the pncA gene, even though it was resistant to PZA and negative for PZase activity. Three isolates had adenine to guanine point mutations in the -11 upstream region, making this the most common type of pncA mutations in this study, with at least two different RFLP patterns. Conclusion: These data help in the understanding of the molecular basis of PZA resistance. An adenine to guanine point mutation in the -11 upstream region was the most common type of pncA mutation in our isolates. The results of pncA mutation analyses should be carefully interpreted for epidemiologic purposes.

AB - Background: Pyrazinamide (PZA) is among the first-line drugs for the treatment of tuberculosis. In vitro, it kills semidormant mycobacteria only at low pH. The purpose of this study was to compare PZA resistance with pyrazinamidase (PZase) activity and the genotype to better understand the molecular basis of PZA resistance and to expand the profile of pncA mutations worldwide. Results: Of the 28 tested strains of Mycobacterium tuberculosis, 6 were susceptible to PZA and positive for PZase activity and had no pncA mutations. Twenty-one strains were resistant to PZA and negative for PZase activity and had mutations in the pncA gene, including 15 point mutations, 5 insertions, and 2 deletions. One strain had no mutation in the pncA gene, even though it was resistant to PZA and negative for PZase activity. Three isolates had adenine to guanine point mutations in the -11 upstream region, making this the most common type of pncA mutations in this study, with at least two different RFLP patterns. Conclusion: These data help in the understanding of the molecular basis of PZA resistance. An adenine to guanine point mutation in the -11 upstream region was the most common type of pncA mutation in our isolates. The results of pncA mutation analyses should be carefully interpreted for epidemiologic purposes.

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