Polarization microscope by single polarization modulation method: Novel instrument for visualizing cytoskeleton

In Hee Shin, Sang Mo Shin, Namhui Ahn, Dug Young Kim

Research output: Contribution to journalConference article

Abstract

A novel and simplest polarization microscope by single polarization modulation method to visualize cytoskeletal structure in bio-cells have been developed. The polarization microscope visualizes cytoskeletal structure based on birefringence characteristics of cells. In the polarization microscope, a series of intensity images according to single modulation of rotating linear polarizer were obtained to visualize cytoskeletal structure. Phase retardation and principal axis orientation were calculated by analytic data processing from the acquired intensity images. Phase retardation and principal axis orientation of breast cancer cells, MCF 7 and MDA MB 231 were measured and visualized as their birefringence characteristics by the polarization microscope. Phase retardation sensitivity of the polarization microscope system also was measured in this paper.

Original languageEnglish
Article number71821Y
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7182
DOIs
Publication statusPublished - 2009 Jun 1
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII - San Jose, CA, United States
Duration: 2009 Jan 262009 Jan 28

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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