POLTERGEIST and POLTERGEIST-LIKE1 are essential for the maintenance of post-embryonic shoot and root apical meristems as revealed by a partial loss-of-function mutant allele of pll1 in Arabidopsis

Sang Kee Song, Young Bin Yun, Myeong Min Lee

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1 Citation (Scopus)

Abstract

Background: POLTERGEIST (POL) and POL-LIKE1 (PLL1) encoding related protein phosphatase 2Cs are essential for the establishment of both shoot and root meristems during embryogenesis. As the strong pol pll1 are seedling-lethal due to the lack of hypocotyl vasculature, the roles of POL/PLL1 for the post-embryonic development is difficult to be assessed. Objective: To prepare a weak pol pll1 double mutant that are able to produce post-embryonic organs. Methods: Several T-DNA insertion mutants of pll1 were crossed to pol-6 for the preparation of weak pol pll1. To understand the epistatic interactions between POL/PLL1 and CLAVATAs, the phenotypes of clvs pol pll1 were assessed and the expression patterns of stem cell markers were examined in pol pll1. POLpro:PLL1-GFP expression was examined during the embryogenesis with confocal microscopy. Results: We isolated a pll1-3 (S544N) allele and prepared a weak pol-6 pll1-3. About 5% of pol-6 pll1-3 seedlings continued the post-embryonic development displaying short roots with reduced root meristem, wuschel-like adventitious phyllotaxis, and defective flowers lacking carpel. The clv1, clv2, and clv3 phenotypes led by enlarged shoot meristems were almost completely suppressed in the pol-6 pll1-3. POL/PLL1 were required for the indeterminate floral organ development displayed by agamous. PLL1-GFP asymmetrically localized in the shootward sides of columella cells and increased the size of distal root meristem region by enhancing the WUS-RELATED HOMEOBOX 5 expression suggesting that PLL1 might provide the stem cells and progenies with proper positional information for the asymmetric cell divisions. Conclusion: Together, POL/PLL1 are required for the maintenance of stem cell pools for the post-embryonic development in Arabidopsis.

Original languageEnglish
Pages (from-to)107-116
Number of pages10
JournalGenes and Genomics
Volume42
Issue number1
DOIs
Publication statusPublished - 2020 Jan 1

Bibliographical note

Funding Information:
This study was supported by a Grant from Woo Jang Choon project (Grant number, PJ01093905 to MML) of the Rural Development Administration, Republic of Korea., research funds from Chosun University 2014 (K206888001) and 2015 (K206888002) to S.S.

Funding Information:
This study was supported by a Grant from Woo Jang Choon project (Grant number, PJ01093905 to MML) of the Rural Development Administration, Republic of Korea., research funds from Chosun University 2014 (K206888001) and 2015 (K206888002) to S.S. ACR4 ARABIDOPSIS CRINKLY4 AG AGAMOUS AS ASYMMETRIC LEAVES BAM BARELY ANY MERISTEM CLE CLV3/ENDOSPERM SURROUNDING REGION CLV CLAVATA CRN CORYNE CSC Columella stem cell CUC CUP SHAPED COTYLEDON OC Organizing center PLL1 POLTERGEIST - LIKE1 POL POLTERGEIST QC Quiescent center STM SHOOT MERISTEMLESS WOX WUS - RELATED HOMEOBOX WUS WUSCHEL

Publisher Copyright:
© 2019, The Genetics Society of Korea.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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