Poly(ethylene glycol) hydrogel microparticles containing enzyme-fluorophore conjugates for the detection of organophosphorus compounds

Youngsik Lee, Dongkil Choi, Won Gun Koh, Bumsang Kim

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

For the first step in the development of a microanalysis device to detect organophosphorus compounds, PEG hydrogel microparticles containing conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) as an optical biosensor were fabricated by a dispersion photopolymerization. Using the dual emission property of pH-sensitive SNAFL-1, changes in the microenvironment pH due to enzyme-catalyzed reactions with the analyte of interest were determined fluorescently and used to detect the analyte. The activity and sensitivity of the enzyme and pH-sensitive fluorophore were maintained when they were conjugated and encapsulated within the PEG hydrogel microparticles. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates was affected by the concentration of a model organophosphorus compound (paraoxon) in the range of 1-20 μmol. These results indicate that the PEG microparticles containing AChE-SNAFL conjugates prepared in this study have a possibility to be used as an optical biosensor to detect organophosphorus compounds.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume137
Issue number1
DOIs
Publication statusPublished - 2009 Mar 28

Fingerprint

Organophosphorus Compounds
Fluorophores
Hydrogel
microparticles
Hydrogels
Polyethylene glycols
enzymes
glycols
ethylene
Enzymes
bioinstrumentation
Biosensors
Paraoxon
Photopolymerization
Microanalysis
microanalysis
sensitivity

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

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abstract = "For the first step in the development of a microanalysis device to detect organophosphorus compounds, PEG hydrogel microparticles containing conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) as an optical biosensor were fabricated by a dispersion photopolymerization. Using the dual emission property of pH-sensitive SNAFL-1, changes in the microenvironment pH due to enzyme-catalyzed reactions with the analyte of interest were determined fluorescently and used to detect the analyte. The activity and sensitivity of the enzyme and pH-sensitive fluorophore were maintained when they were conjugated and encapsulated within the PEG hydrogel microparticles. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates was affected by the concentration of a model organophosphorus compound (paraoxon) in the range of 1-20 μmol. These results indicate that the PEG microparticles containing AChE-SNAFL conjugates prepared in this study have a possibility to be used as an optical biosensor to detect organophosphorus compounds.",
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Poly(ethylene glycol) hydrogel microparticles containing enzyme-fluorophore conjugates for the detection of organophosphorus compounds. / Lee, Youngsik; Choi, Dongkil; Koh, Won Gun; Kim, Bumsang.

In: Sensors and Actuators, B: Chemical, Vol. 137, No. 1, 28.03.2009, p. 209-214.

Research output: Contribution to journalArticle

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AB - For the first step in the development of a microanalysis device to detect organophosphorus compounds, PEG hydrogel microparticles containing conjugates of enzyme (AChE) and pH-sensitive fluorophore (SNAFL-1) as an optical biosensor were fabricated by a dispersion photopolymerization. Using the dual emission property of pH-sensitive SNAFL-1, changes in the microenvironment pH due to enzyme-catalyzed reactions with the analyte of interest were determined fluorescently and used to detect the analyte. The activity and sensitivity of the enzyme and pH-sensitive fluorophore were maintained when they were conjugated and encapsulated within the PEG hydrogel microparticles. The fluorescent response of the PEG microparticles containing AChE-SNAFL conjugates was affected by the concentration of a model organophosphorus compound (paraoxon) in the range of 1-20 μmol. These results indicate that the PEG microparticles containing AChE-SNAFL conjugates prepared in this study have a possibility to be used as an optical biosensor to detect organophosphorus compounds.

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