Poly(ethylene glycol) hydrogel microstructures encapsulating living cells

Won-Gun Koh, Alexander Revzin, Michael V. Pishko

Research output: Contribution to journalArticle

202 Citations (Scopus)

Abstract

We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 μm in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 μm allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.

Original languageEnglish
Pages (from-to)2459-2462
Number of pages4
JournalLangmuir
Volume18
Issue number7
DOIs
Publication statusPublished - 2002 Apr 2

Fingerprint

encapsulating
Hydrogel
Hydrogels
Polyethylene glycols
glycols
ethylene
Cells
microstructure
Microstructure
Encapsulation
cells
Photolithography
photolithography
viability
Microfluidics
Assays
Substrates

All Science Journal Classification (ASJC) codes

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Koh, Won-Gun ; Revzin, Alexander ; Pishko, Michael V. / Poly(ethylene glycol) hydrogel microstructures encapsulating living cells. In: Langmuir. 2002 ; Vol. 18, No. 7. pp. 2459-2462.
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Poly(ethylene glycol) hydrogel microstructures encapsulating living cells. / Koh, Won-Gun; Revzin, Alexander; Pishko, Michael V.

In: Langmuir, Vol. 18, No. 7, 02.04.2002, p. 2459-2462.

Research output: Contribution to journalArticle

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