PolyHEMA soft lithograpy for selective cell seeding, migration blocking, and high-throughput suspension cell culture

Patrick N. Ingram, Yu Chih Chen, Euisik Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

In this work, we report a novel method for patterning poly-2-hydroxyethyl methacrylate (PolyHEMA) utilizing a variation of standard polydimethylsiloxane (PDMS) soft lithographic stamping. We have demonstrated precise micronscale patterning of polyHEMA on both glass and polystyrene substrates, facilitating fabrication of large scale polyHEMA cell culture arrays. These large scale microwell arrays were used to capture and culture single SUM159 breast cancer cells in both adherent and non-adherent environments. The unique chemical composition of polyHEMA allows its use in migration blocking, suspension culture, and even, if so desired, as a master for subsequent lithographic processing.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1451-1453
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume3

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Cell culture
Suspensions
Throughput
Stamping
Polystyrenes
Polydimethylsiloxane
Cells
Fabrication
Glass
Substrates
Processing
Chemical analysis
hydroxyethyl methacrylate
baysilon

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Ingram, P. N., Chen, Y. C., & Yoon, E. (2013). PolyHEMA soft lithograpy for selective cell seeding, migration blocking, and high-throughput suspension cell culture. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1451-1453). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3). Chemical and Biological Microsystems Society.
Ingram, Patrick N. ; Chen, Yu Chih ; Yoon, Euisik. / PolyHEMA soft lithograpy for selective cell seeding, migration blocking, and high-throughput suspension cell culture. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 1451-1453 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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abstract = "In this work, we report a novel method for patterning poly-2-hydroxyethyl methacrylate (PolyHEMA) utilizing a variation of standard polydimethylsiloxane (PDMS) soft lithographic stamping. We have demonstrated precise micronscale patterning of polyHEMA on both glass and polystyrene substrates, facilitating fabrication of large scale polyHEMA cell culture arrays. These large scale microwell arrays were used to capture and culture single SUM159 breast cancer cells in both adherent and non-adherent environments. The unique chemical composition of polyHEMA allows its use in migration blocking, suspension culture, and even, if so desired, as a master for subsequent lithographic processing.",
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Ingram, PN, Chen, YC & Yoon, E 2013, PolyHEMA soft lithograpy for selective cell seeding, migration blocking, and high-throughput suspension cell culture. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 3, Chemical and Biological Microsystems Society, pp. 1451-1453, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.

PolyHEMA soft lithograpy for selective cell seeding, migration blocking, and high-throughput suspension cell culture. / Ingram, Patrick N.; Chen, Yu Chih; Yoon, Euisik.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 1451-1453 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Ingram PN, Chen YC, Yoon E. PolyHEMA soft lithograpy for selective cell seeding, migration blocking, and high-throughput suspension cell culture. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 1451-1453. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).