Polyhydroxy butyrate production by Acinetobacter junii BP25, Aeromonas hydrophila ATCC 7966, and their co-culture using a feast and famine strategy

Parthiban Anburajan, A. Naresh Kumar, Poorna Chandrika Sabapathy, Gi Beom Kim, Roent Dune Cayetano, Jeong Jun Yoon, Gopalakrishnan Kumar, Sang Hyoun Kim

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The study aimed to evaluate biopolymer production using two bacterial strains, Acinetobacter junii BP25 and Aeromonas hydrophila ATCC 7966, and their co-culture. Batch experiments were evaluated using acetate and butyrate as carbon sources in feast and famine strategy. Feast phase was studied using carbon, nitrates and phosphate in the ratio of 100:8:1 and famine phase was limited with the phosphate and nitrates. Co-culture resulted in highest specific growth rate (0.30 h−1) in the feast phase and the famine phase accounted the maximum polyhydroxybutyrate (PHB) accumulation (2.46 g PHB/L), followed by Acinetobacter junii BP25 (0.25 h−1 and 1.82 g PHB/L) and Aeromonas hydrophila ATCC 7966 (0.17 h−1 and 1.12 g PHB/L). Fourier-transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR) structural analysis confirmed as PHB. PHB production using the co-culture could be integrated with biohydrogen process using volatile fatty acids (VFA) as a carbon source in the biorefinery framework.

Original languageEnglish
Article number122062
JournalBioresource technology
Publication statusPublished - 2019 Dec


All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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