Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a south Korean population

Isabela N. Rôças, Il Young Jung, Chan Young Lee, José F. Siqueira

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94 Citations (Scopus)

Abstract

A large body of evidence indicates that microorganisms are the primary causative agents of endodontic treatment failures. This study intended to assess the occurrence of nine putative endodontic pathogens in root-filled teeth associated with periradicular lesions in a South Korean population using a culture-independent molecular approach. Fourteen root-filled teeth with persistent periradicular diseases were selected for retreatment. After removal of the root canal filling, the canals were sampled, and a polymerase chain reaction assay using taxon-specific oligonucleotide primers was used for microbial detection. Bacteria were present in all cases, as revealed by amplification using ubiquitous 16S rDNA primers. The most frequently detected taxon was Enterococcus faecalis (64%), followed by Streptococcus spp. (21%) and Tannerella forsythensis (14%). The results of this study using a highly sensitive identification method are concurrent with those from other geographical locations using diverse identification methods in that E. faecalis is the main species found in cases of root-filled teeth associated with periradicular lesions.

Original languageEnglish
Pages (from-to)504-508
Number of pages5
JournalJournal of endodontics
Volume30
Issue number7
DOIs
Publication statusPublished - 2004 Jul

Bibliographical note

Funding Information:
Supported in part by grants from CNPq and FAPERJ, Brazilian Governmental Institutions.

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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