Polyubiquitin chain-dependent protein degradation in TRIM30 cytoplasmic bodies

Un Yung Choi, Won Young Choi, Ji Yeon Hur, Young Joon Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Viral infection induces numerous tripartite motif (TRIM) proteins to control antiviral immune signaling and viral replication. Particularly, SPRY-containing TRIM proteins are found only in vertebrates and they control target protein degradation by their RING-finger and SPRY domains, and proper cytoplasmic localization. To understand TRIM30 function, we analyzed its localization pattern and putative roles of its RING-finger and SPRY domains. We found that TRIM30 is located in actin-mediated cytoplasmic bodies and produces colocalized ubiquitin chains in SPRY domain-and RING-finger domain-dependent ways that are degraded by autophagy and the proteasome. These results suggest a TRIM protein-dependent degradation mechanism by cytoplasmic body formation with actin networks.

Original languageEnglish
Article numbere159
JournalExperimental and Molecular Medicine
Volume47
Issue number4
DOIs
Publication statusPublished - 2015 Apr

Bibliographical note

Funding Information:
This research was supported by the Global Research Laboratory Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning (MEST; K20705000006-12A0500-00610 to Y-JK), the Bio and Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning (MEST; 2012028272 to Y-JK), the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (grant number: HI13C08470200), the Introduced Innovative R&D Team Leadership of Guangdong Province, People’s Republic of China.

Publisher Copyright:
© 2015 KSBMB.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

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