Pore Diameter of Mesoporous Silica Modulates Oxidation of H2O2-Sensing Chromophore in a Porous Matrix

Jiayu Leong, Yongbeom Seo, Sang Hyon Chu, Cheol Park, Eun Je Jeon, Seung Woo Cho, Yi Yan Yang, Luisa A. Dipietro, Dong Hyun Kim, Hyunjoon Kong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2) is an attractive chemical because of its bleaching properties in paper and pulp industry and as a disinfectant in the food, water, and medical industries. However, it is important to monitor the residual H2O2 level after its usage and prevent any unintended health problems or chemical reactions. Most H2O2 sensors often utilize fluorophores or electrical circuitry that requires an additional irradiation or a digital display. To this end, this study presents a 3,3′,5,5′-tetramethylbenzidine (TMB)/horseradish peroxidase (HRP)-loaded patch that alerts the presence of high H2O2 levels by generating a visible blue color. We hypothesized that water-insoluble TMB immobilized within mesoporous silica particles of proper pore diameter and structure would act as a colorimetric indicator through the H2O2-mediated oxidation within a cross-linked patch. We examined this hypothesis by immobilizing TMB molecules in mesoporous silica particles with 2 and 7 nm diameter cylindrical pores as well as on nonporous silica particles. Then, we loaded these TMB-silica particles and HRP in a porous alginate patch via sequential in situ cross-linking reaction and lyophilization. In the presence of 25-5000 μM H2O2, which simulate H2O2 concentrations found in residual disinfecting fluids, the patch loaded with TMB-mesoporous silica particles with 7 nm diameter pores generated a distinct blue color with varying intensities depending on the H2O2 concentration. The design principles demonstrated in this study should be applicable to a broad array of sensors to be integrated into a moldable, three-dimensional matrix.

Original languageEnglish
Pages (from-to)11242-11252
Number of pages11
JournalLangmuir
Volume34
Issue number38
DOIs
Publication statusPublished - 2018 Sep 25

Fingerprint

Chromophores
Silicon Dioxide
chromophores
Silica
silicon dioxide
porosity
Oxidation
oxidation
matrices
Horseradish Peroxidase
colloiding
antiseptics
industries
Color
color
Disinfectants
Fluorophores
Water
Paper and pulp industry
sensors

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Leong, Jiayu ; Seo, Yongbeom ; Chu, Sang Hyon ; Park, Cheol ; Jeon, Eun Je ; Cho, Seung Woo ; Yang, Yi Yan ; Dipietro, Luisa A. ; Kim, Dong Hyun ; Kong, Hyunjoon. / Pore Diameter of Mesoporous Silica Modulates Oxidation of H2O2-Sensing Chromophore in a Porous Matrix. In: Langmuir. 2018 ; Vol. 34, No. 38. pp. 11242-11252.
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abstract = "Hydrogen peroxide (H2O2) is an attractive chemical because of its bleaching properties in paper and pulp industry and as a disinfectant in the food, water, and medical industries. However, it is important to monitor the residual H2O2 level after its usage and prevent any unintended health problems or chemical reactions. Most H2O2 sensors often utilize fluorophores or electrical circuitry that requires an additional irradiation or a digital display. To this end, this study presents a 3,3′,5,5′-tetramethylbenzidine (TMB)/horseradish peroxidase (HRP)-loaded patch that alerts the presence of high H2O2 levels by generating a visible blue color. We hypothesized that water-insoluble TMB immobilized within mesoporous silica particles of proper pore diameter and structure would act as a colorimetric indicator through the H2O2-mediated oxidation within a cross-linked patch. We examined this hypothesis by immobilizing TMB molecules in mesoporous silica particles with 2 and 7 nm diameter cylindrical pores as well as on nonporous silica particles. Then, we loaded these TMB-silica particles and HRP in a porous alginate patch via sequential in situ cross-linking reaction and lyophilization. In the presence of 25-5000 μM H2O2, which simulate H2O2 concentrations found in residual disinfecting fluids, the patch loaded with TMB-mesoporous silica particles with 7 nm diameter pores generated a distinct blue color with varying intensities depending on the H2O2 concentration. The design principles demonstrated in this study should be applicable to a broad array of sensors to be integrated into a moldable, three-dimensional matrix.",
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Leong, J, Seo, Y, Chu, SH, Park, C, Jeon, EJ, Cho, SW, Yang, YY, Dipietro, LA, Kim, DH & Kong, H 2018, 'Pore Diameter of Mesoporous Silica Modulates Oxidation of H2O2-Sensing Chromophore in a Porous Matrix', Langmuir, vol. 34, no. 38, pp. 11242-11252. https://doi.org/10.1021/acs.langmuir.8b00957

Pore Diameter of Mesoporous Silica Modulates Oxidation of H2O2-Sensing Chromophore in a Porous Matrix. / Leong, Jiayu; Seo, Yongbeom; Chu, Sang Hyon; Park, Cheol; Jeon, Eun Je; Cho, Seung Woo; Yang, Yi Yan; Dipietro, Luisa A.; Kim, Dong Hyun; Kong, Hyunjoon.

In: Langmuir, Vol. 34, No. 38, 25.09.2018, p. 11242-11252.

Research output: Contribution to journalArticle

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AU - Leong, Jiayu

AU - Seo, Yongbeom

AU - Chu, Sang Hyon

AU - Park, Cheol

AU - Jeon, Eun Je

AU - Cho, Seung Woo

AU - Yang, Yi Yan

AU - Dipietro, Luisa A.

AU - Kim, Dong Hyun

AU - Kong, Hyunjoon

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